Anti-ARHGAP25 Antibody (CAB1223)
- SKU:
- CAB1223
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ARHGAP25 Antibody |
| Antibody SKU: | CAB1223 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_001159748.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | COS-7, OVCAR3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_001159748.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSLG QSAC LFLS IARS RSVM TGEQ MAAF HPSS TPNP LERP IKMG WLKK QRSI VKNW QQRY FVLR AQQL YYYK DEED TKPQ GCMY LPGC TIKE IATN PEEA GKFV FEII PASW DQNR MGQD SYVL MASS QAEM EEWV KFLR RVAG TPCG AVFG QRLD ETVA YEQK FGPH LVPI LVEK CAEF ILEH GRNE EGIF RLPG QDNL VKQL RDAF DAGE RPSF DRDT DVHT VASL LKLY LRDL PEPV VPWS QYEG FLLC GQLT NADE AKAQ QELM KQLS ILPR DNYS LLSY ICRF LHEI QLNC AVNK MSVD NLAT VIGV NLIR SKVE DPAV IMRG TPQI QRVM TMMI RDHE VLFP KSKD IPLS PPAQ KNDP KKAP VA |
| Gene ID: | 9938 |
| Uniprot: | P42331 |
| Cellular Location: | |
| Calculated MW: | 51kDa/69kDa/72kDa/73kDa |
| Observed MW: | 70kDa |
| Synonyms: | ARHGAP25, HEL-S-308, KAIA0053 |
| Background: |
| UniProt Protein Function: | ARHGAP25: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GAPs, Rac/Rho; GAPs Chromosomal Location of Human Ortholog: 2p13.3 Cellular Component: cytosol Molecular Function:GTPase activator activity Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity |
| NCBI Summary: | ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | P42331 |
| NCBI GenInfo Identifier: | 238054314 |
| NCBI Gene ID: | 9938 |
| NCBI Accession: | P42331.2 |
| UniProt Secondary Accession: | P42331,Q8IXQ2, A8K2Y1, B7Z498, E9PFQ7, G5E9G2, |
| UniProt Related Accession: | P42331 |
| Molecular Weight: | 645 |
| NCBI Full Name: | Rho GTPase-activating protein 25 |
| NCBI Synonym Full Names: | Rho GTPase activating protein 25 |
| NCBI Official Symbol: | ARHGAP25 |
| NCBI Official Synonym Symbols: | KAIA0053; HEL-S-308 |
| NCBI Protein Information: | rho GTPase-activating protein 25; epididymis secretory protein Li 308; rho-type GTPase-activating protein 25 |
| UniProt Protein Name: | Rho GTPase-activating protein 25 |
| UniProt Synonym Protein Names: | Rho-type GTPase-activating protein 25 |
| UniProt Gene Name: | ARHGAP25 |
| UniProt Entry Name: | RHG25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARHGAP25 antibody (CAB1223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human kidney using ARHGAP25 antibody (CAB1223) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human gastric using ARHGAP25 antibody (CAB1223) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of Jurkat cells using ARHGAP25 Polyclonal Antibody (CAB1223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ARHGAP25 Polyclonal Antibody (CAB1223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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