Anti-ARAP1 항체 (CAB10466)

Antibody Name:	Anti-ARAP1 Antibody
Antibody SKU:	CAB10466
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human ARAP1 (NP_001128662.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, LO2, HeLa, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human ARAP1 (NP_001128662.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SVDE EELR KQRE EITA IVKM RVAG TASG TQHA GDFI CTVY LEEK KAET EQHI KVPA SMTA EELT LEIL DRRN VGIR EKDY WTCF EVNE REEA ERPL HFAE KVLP ILHG LGTD SHLV VKKH QAME AMLL YLAS RVGD TKHG MMKF REDR SLLG LGLP SGGF HDRY FILN SSCL RLYK EVRS HRPE KEWP IKSL KVYL GVKK KLRP PTCW GFTV VHET EKHE KQQW YLCC DTQM ELRE WFAT FLFV QHDG LVWP SEPS RVSR AVPE VRLG SVSL IPLR GSEN EMRR SVAA FTAD PLSL LRNV
Gene ID:	116985
Uniprot:	Q96P48
Cellular Location:	Cell membrane, Cytoplasm, Golgi apparatus, Golgi stack membrane, Peripheral membrane protein
Calculated MW:	77kDa/127kDa/135kDa/136kDa/160kDa/162kDa
Observed MW:	190kDa

Synonyms:

ARAP1, CENTD2

Background:

The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3, 4, 5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CENTD2: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GAP; GAP, ARF; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11q13.4 Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm; plasma membrane Molecular Function:GTPase activator activity; phosphatidylinositol-3,4,5-triphosphate binding; protein binding Biological Process: actin filament reorganization during cell cycle; negative regulation of stress fiber formation; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of filopodium formation; positive regulation of GTPase activity; regulation of cell motility; regulation of cell shape; regulation of small GTPase mediated signal transduction
NCBI Summary:	The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
UniProt Code:	Q96P48
NCBI GenInfo Identifier:	226694321
NCBI Gene ID:	116985
NCBI Accession:	Q96P48.3
UniProt Secondary Accession:	Q96P48,O94879, Q4LDD5, Q59FI7, Q6PHS3, Q8WU51, Q96HP6 Q96L71, A3KLL7, B2RTS2,
UniProt Related Accession:	Q96P48
Molecular Weight:	162kDa
NCBI Full Name:	Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
NCBI Synonym Full Names:	ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
NCBI Official Symbol:	ARAP1
NCBI Official Synonym Symbols:	CENTD2
NCBI Protein Information:	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
UniProt Protein Name:	Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
UniProt Synonym Protein Names:	Centaurin-delta-2; Cnt-d2
Protein Family:	Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein
UniProt Gene Name:	ARAP1

Western blot analysis of extracts of various cell lines, using ARAP1 antibody (CAB10466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)