Anti-Aquaporin 4 Antibody (CAB2887)
- SKU:
- CAB2887
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-Aquaporin 4 Antibody |
| Antibody SKU: | CAB2887 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 244-323 of human Aquaporin 4 (NP_001641.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-549, 293T, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 244-323 of human Aquaporin 4 (NP_001641.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AGGL YEYV FCPD VEFK RRFK EAFS KAAQ QTKG SYME VEDN RSQV ETDD LILK PGVV HVID VDRG EEKK GKDQ SGEV LSSV |
| Gene ID: | 361 |
| Uniprot: | P55087 |
| Cellular Location: | Membrane, Multi-pass membrane protein |
| Calculated MW: | 32kDa/34kDa |
| Observed MW: | 36kDa |
| Synonyms: | AQP4, MIWC, WCH4, aquaporin-4 |
| Background: | This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. |
| UniProt Protein Function: | AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described |
| UniProt Protein Details: | Protein type:Transporter, aquaporin family; Membrane protein, integral; Channel, misc.; Membrane protein, multi-pass; Transporter Chromosomal Location of Human Ortholog: 18q11.2-q12.1 Cellular Component: basolateral plasma membrane; cytoplasm; external side of plasma membrane; integral to membrane; plasma membrane Molecular Function:glycerol channel activity; water channel activity; water transporter activity Biological Process: glycerol transport; multicellular organismal water homeostasis; renal water homeostasis; transmembrane transport; transport; water transport |
| NCBI Summary: | This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P55087 |
| NCBI GenInfo Identifier: | 2506859 |
| NCBI Gene ID: | 361 |
| NCBI Accession: | P55087.2 |
| UniProt Secondary Accession: | P55087,P78564, |
| UniProt Related Accession: | P55087 |
| Molecular Weight: | 32,300 Da |
| NCBI Full Name: | Aquaporin-4 |
| NCBI Synonym Full Names: | aquaporin 4 |
| NCBI Official Symbol: | AQP4 |
| NCBI Official Synonym Symbols: | MIWC; WCH4 |
| NCBI Protein Information: | aquaporin-4 |
| UniProt Protein Name: | Aquaporin-4 |
| UniProt Synonym Protein Names: | Mercurial-insensitive water channel; MIWC; WCH4 |
| Protein Family: | Aquaporin |
| UniProt Gene Name: | AQP4 |
| UniProt Entry Name: | AQP4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Aquaporin 4 antibody (CAB2887) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat lung using Aquaporin 4 antibody (CAB2887) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using Aquaporin 4 antibody (CAB2887) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using Aquaporin 4 antibody (CAB2887) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of mouse brain using Aquaporin 4 Polyclonal Antibody (CAB2887) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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