Anti-APRT Antibody (CAB13946)
- SKU:
- CAB13946
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-APRT Antibody |
| Antibody SKU: | CAB13946 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, A-549, K-562, HeLa, Mouse intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADS ELQL VEQR IRSF PDFP TPGV VFRD ISPV LKDP ASFR AAIG LLAR HLKA THGG RIDY IAGL DSRG FLFG PSLA QELG LGCV LIRK RGKL PGPT LWAS YSLE YGKA ELEI QKDA LEPG QRVV VVDD LLAT GGTM NAAC ELLG RLQA EVLE CVSL VELT SLKG REKL APVP FFSL LQYE |
| Gene ID: | 353 |
| Uniprot: | P07741 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 14kDa/19kDa |
| Observed MW: | 19-22kDa |
| Synonyms: | APRT, AMP, APRTD |
| Background: | Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2, 8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; Transferase; EC 2.4.2.7 Chromosomal Location of Human Ortholog: 16q24 Cellular Component: nucleoplasm; cytoplasm; cytosol Molecular Function:adenine binding; adenine phosphoribosyltransferase activity; AMP binding Biological Process: grooming behavior; lactation; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine salvage; purine base metabolic process Disease: Adenine Phosphoribosyltransferase Deficiency |
| NCBI Summary: | Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07741 |
| NCBI GenInfo Identifier: | 114074 |
| NCBI Gene ID: | 353 |
| NCBI Accession: | P07741.2 |
| UniProt Secondary Accession: | P07741,Q3KP55, Q68DF9, G5E9J2, |
| UniProt Related Accession: | P07741 |
| Molecular Weight: | 14,557 Da |
| NCBI Full Name: | Adenine phosphoribosyltransferase |
| NCBI Synonym Full Names: | adenine phosphoribosyltransferase |
| NCBI Official Symbol: | APRT |
| NCBI Official Synonym Symbols: | AMP; APRTD |
| NCBI Protein Information: | adenine phosphoribosyltransferase; AMP diphosphorylase; AMP pyrophosphorylase; transphosphoribosidase |
| UniProt Protein Name: | Adenine phosphoribosyltransferase |
| Protein Family: | Probable adenine phosphoribosyltransferase |
| UniProt Gene Name: | APRT |
| UniProt Entry Name: | APT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using APRT antibody (CAB13946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human prostate using APRT Antibody (CAB13946) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using APRT Antibody (CAB13946) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using APRT Rabbit pAb (CAB13946) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using APRT Rabbit pAb (CAB13946) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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