Anti-Apolipoprotein A-IV Antibody (CAB9792)
- SKU:
- CAB9792
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Apolipoprotein A-IV Antibody |
| Antibody SKU: | CAB9792 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 227-396 of human APOA4 (NP_000473.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 227-396 of human APOA4 (NP_000473.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AQDT QEKL NHQL EGLT FQMK KNAE ELKA RISA SAEE LRQR LAPL AEDV RGNL RGNT EGLQ KSLA ELGG HLDQ QVEE FRRR VEPY GENF NKAL VQQM EQLR QKLG PHAG DVEG HLSF LEKD LRDK VNSF FSTF KEKE SQDK TLSL PELE QQQE QQQE QQQE QVQM LAPL ES |
| Gene ID: | 337 |
| Uniprot: | P06727 |
| Cellular Location: | Secreted |
| Calculated MW: | 45kDa |
| Observed MW: | 45kDa |
| Synonyms: | APOA4 |
| Background: | Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. |
| UniProt Protein Function: | APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family. |
| UniProt Protein Details: | Protein type:Cell adhesion; Endoplasmic reticulum; Lipid-binding; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: cell surface; chylomicron; cytosol; early endosome; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space Molecular Function:antioxidant activity; cholesterol binding; cholesterol transporter activity; copper ion binding; lipid binding; lipid transporter activity; phosphatidylcholine binding; protein binding; protein homodimerization activity Biological Process: cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; hydrogen peroxide catabolic process; innate immune response in mucosa; leukocyte adhesion; lipid homeostasis; lipid transport; lipoprotein metabolic process; multicellular organismal lipid catabolic process; neuron projection regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; protein-lipid complex assembly; regulation of cholesterol transport; regulation of intestinal cholesterol absorption; removal of superoxide radicals; response to lipid hydroperoxide; retinoid metabolic process; reverse cholesterol transport |
| NCBI Summary: | Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P06727 |
| NCBI GenInfo Identifier: | 93163358 |
| NCBI Gene ID: | 337 |
| NCBI Accession: | P06727.3 |
| UniProt Secondary Accession: | P06727,Q14CW8, Q6Q787, A8MSL6, |
| UniProt Related Accession: | P06727 |
| Molecular Weight: | |
| NCBI Full Name: | Apolipoprotein A-IV |
| NCBI Synonym Full Names: | apolipoprotein A4 |
| NCBI Official Symbol: | APOA4 |
| NCBI Protein Information: | apolipoprotein A-IV |
| UniProt Protein Name: | Apolipoprotein A-IV |
| UniProt Synonym Protein Names: | Apolipoprotein A4 |
| Protein Family: | Apolipoprotein |
| UniProt Gene Name: | APOA4 |
 | Western blot analysis of extracts of various cell lines, using APOA4 antibody (CAB9792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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