Anti-APLP2 Antibody (CAB5455)
- SKU:
- CAB5455
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-APLP2 Antibody |
| Antibody SKU: | CAB5455 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-450 of human APLP2 (NP_001135750.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse heart, Mouse testis, Rat brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-450 of human APLP2 (NP_001135750.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RADM DQFT ASIS ETPV DVRV SSEE SEEI PPFH PFHP FPAL PENE GSGV GEQD GGLI GAEE KVIN SKNK VDEN MVID ETLD VKEM IFNA ERVG GLEE ERES VGPL REDF SLS |
| Gene ID: | 334 |
| Uniprot: | Q06481 |
| Cellular Location: | Cell membrane, Nucleus, Single-pass type I membrane protein |
| Calculated MW: | 59kDa/79kDa/80kDa/85kDa/86kDa/87kDa |
| Observed MW: | 87KDa |
| Synonyms: | APLP2, APLP-2, APPH, APPL2, CDEBP |
| Background: | This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | APLP2: May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts. Belongs to the APP family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Receptor, misc.; Cell surface; DNA-binding; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q24 Cellular Component: membrane; nucleus; plasma membrane; platelet alpha granule membrane Molecular Function:identical protein binding; protein binding Biological Process: platelet degranulation |
| NCBI Summary: | This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q06481 |
| NCBI GenInfo Identifier: | 1703344 |
| NCBI Gene ID: | 334 |
| NCBI Accession: | Q06481.2 |
| UniProt Secondary Accession: | Q06481,Q13861, Q14594, Q14662, Q71U10, Q7M4L3, Q9BT36 B3KXX9, H7BXI4, |
| UniProt Related Accession: | Q06481 |
| Molecular Weight: | 87,215 Da |
| NCBI Full Name: | Amyloid-like protein 2 |
| NCBI Synonym Full Names: | amyloid beta precursor like protein 2 |
| NCBI Official Symbol: | APLP2 |
| NCBI Official Synonym Symbols: | APPH; APPL2; CDEBP; APLP-2 |
| NCBI Protein Information: | amyloid-like protein 2 |
| UniProt Protein Name: | Amyloid-like protein 2 |
| UniProt Synonym Protein Names: | APPH; Amyloid protein homolog; CDEI box-binding protein; CDEBP |
| Protein Family: | Amyloid-like protein |
| UniProt Gene Name: | APLP2 |
| UniProt Entry Name: | APLP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using APLP2 Polyclonal Antibody (CAB5455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using APLP2 Rabbit pAb (CAB5455) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |