Anti-APEX2 Antibody (CAB16116)

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SKU:
CAB16116
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-APEX2 Antibody
Antibody SKU:CAB16116
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APEX2 (NP_055296.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APEX2 (NP_055296.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLRV VSWN INGI RRPL QGVA NQEP SNCA AVAV GRIL DELD ADIV CLQE TKVT RDAL TEPL AIVE GYNS YFSF SRNR SGYS GVAT FCKD NATP VAAE EGLS GLFA TQNG DVGC YGNM DEFT QEEL RALD SEGR ALLT QHKI RTWE GKEK TLTL INVY CPHA DPGR PERL VFKM RFYR LLQI
Gene ID:27301
Uniprot:Q9UBZ4
Cellular Location:Cytoplasm, Mitochondrion, Nucleus
Calculated MW:57kDa
Observed MW:57kDa
Synonyms:APEX2, APE2, APEXL2, XTH2, ZGRF2
Background:Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:APEX2: Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. Belongs to the DNA repair enzymes AP/ExoA family.
UniProt Protein Details:

Protein type:Lyase; EC 4.2.99.18; Mitochondrial

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: intracellular membrane-bound organelle; mitochondrial inner membrane; nucleolus; nucleus

Molecular Function:DNA-(apurinic or apyrimidinic site) lyase activity; DNA binding; zinc ion binding; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity

Biological Process: base-excision repair; DNA catabolic process, exonucleolytic; cell cycle; DNA catabolic process, endonucleolytic; DNA recombination

NCBI Summary:Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]
UniProt Code:Q9UBZ4
NCBI GenInfo Identifier:73921676
NCBI Gene ID:27301
NCBI Accession:Q9UBZ4.1
UniProt Secondary Accession:Q9UBZ4,Q9Y5X7,
UniProt Related Accession:Q9UBZ4
Molecular Weight:57,401 Da
NCBI Full Name:DNA-(apurinic or apyrimidinic site) lyase 2
NCBI Synonym Full Names:APEX nuclease (apurinic/apyrimidinic endonuclease) 2
NCBI Official Symbol:APEX2  
NCBI Official Synonym Symbols:APE2; XTH2; ZGRF2; APEXL2  
NCBI Protein Information:DNA-(apurinic or apyrimidinic site) lyase 2; DNA-(apurinic or apyrimidinic site) lyase 2; AP endonuclease 2; AP endonuclease XTH2; zinc finger, GRF-type containing 2; apurinic/apyrimidinic endonuclease-like 2
UniProt Protein Name:DNA-(apurinic or apyrimidinic site) lyase 2
UniProt Synonym Protein Names:AP endonuclease XTH2; APEX nuclease 2; APEX nuclease-like 2; Apurinic-apyrimidinic endonuclease 2; AP endonuclease 2
Protein Family:Aminopeptidase
UniProt Gene Name:APEX2  
UniProt Entry Name:APEX2_HUMAN
Anti-APEX2 Antibody (CAB16116)Western blot analysis of extracts of various cell lines, using APEX2 antibody (CAB16116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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