Anti-APEH Antibody (CAB13467)
- SKU:
- CAB13467
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-APEH Antibody |
| Antibody SKU: | CAB13467 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, Jurkat, Mouse liver, Mouse kidney, Mouse small intestine, Rat bone marrow |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MERQ VLLS EPEE AAAL YRGL SRQP ALSA ACLG PEVT TQYG GQYR TVHT EWTQ RDLE RMEN IRFC RQYL VFHD GDSV VFAG PAGN SVET RGEL LSRE SPSG TMKA VLRK AGGT GPGE EKQF LEVW EKNR KLKS FNLS ALEK HGPV YEDD CFGC LSWS HSET HLLY VAEK KRPK AESF FQTK ALDV SASD DEIA RLKK PDQA IKGD QFVF YEDW GENM VSKS IPVL CVLD VESG NISV LEGV PENV SPGQ AFWA PGDA GVVF |
| Gene ID: | 327 |
| Uniprot: | P13798 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 81kDa |
| Observed MW: | 80-108kDa |
| Synonyms: | APEH, AARE, ACPH, APH, D3F15S2, D3S48E, DNF15S2, OPH |
| Background: | This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. |
| UniProt Protein Function: | APEH: This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N- acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Belongs to the peptidase S9C family. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.19.1 Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: nuclear membrane; cytoplasm Molecular Function:omega peptidase activity; serine-type endopeptidase activity Biological Process: beta-amyloid metabolic process; proteolysis |
| NCBI Summary: | This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13798 |
| NCBI GenInfo Identifier: | 38258902 |
| NCBI Gene ID: | 327 |
| NCBI Accession: | P13798.4 |
| UniProt Secondary Accession: | P13798,Q9BQ33, Q9P0Y2, |
| UniProt Related Accession: | P13798 |
| Molecular Weight: | 732 |
| NCBI Full Name: | Acylamino-acid-releasing enzyme |
| NCBI Synonym Full Names: | acylaminoacyl-peptide hydrolase |
| NCBI Official Symbol: | APEH |
| NCBI Official Synonym Symbols: | APH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2 |
| NCBI Protein Information: | acylamino-acid-releasing enzyme; acyl-peptide hydrolase; acylaminoacyl-peptidase; oxidized protein hydrolase; N-acylaminoacyl-peptide hydrolase |
| UniProt Protein Name: | Acylamino-acid-releasing enzyme |
| UniProt Synonym Protein Names: | Acyl-peptide hydrolase; APH; Acylaminoacyl-peptidase; Oxidized protein hydrolase; OPH |
| Protein Family: | Acylamino-acid-releasing enzyme |
| UniProt Gene Name: | APEH |
| UniProt Entry Name: | ACPH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using APEH antibody (CAB13467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using APEH antibody (CAB13467). Blue: DAPI for nuclear staining. |
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