Anti-APC Antibody (CAB2818)
- SKU:
- CAB2818
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-APC Antibody |
| Antibody SKU: | CAB2818 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2544-2843 of human APC (NP_000029.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2544-2843 of human APC (NP_000029.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SGTW KREH SKHS SSLP RVST WRRT GSSS SILS ASSE SSEK AKSE DEKH VNSI SGTK QSKE NQVS AKGT WRKI KENE FSPT NSTS QTVS SGAT NGAE SKTL IYQM APAV SKTE DVWV RIED CPIN NPRS GRSP TGNT PPVI DSVS EKAN PNIK DSKD NQAK QNVG NGSV PMRT VGLE NRLN SFIQ VDAP DQKG TEIK PGQN NPVP VSET NESS IVER TPFS SSSS SKHS SPSG TVAA RVTP FNYN PSPR KSSA DSTS ARPS QIPT PVNN NTKK RDSK TDST ESSG TQSP KRHS GSYL VTSV |
| Gene ID: | 324 |
| Uniprot: | P25054 |
| Cellular Location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, adherens junction, cytoskeleton, lamellipodium, ruffle membrane |
| Calculated MW: | 300kDa/311kDa |
| Observed MW: | 290kDa |
| Synonyms: | APC, BTPS2, DP2, DP2.5, DP3, GS, PPP1R46 |
| Background: | This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. |
| UniProt Code: | P25054 |
| NCBI GenInfo Identifier: | 97535708 |
| NCBI Gene ID: | 324 |
| NCBI Accession: | P25054.2 |
| UniProt Secondary Accession: | P25054,Q15162, Q15163, Q93042, D3DT03, |
| UniProt Related Accession: | P25054 |
| Molecular Weight: | 461 |
| NCBI Full Name: | Adenomatous polyposis coli protein |
| NCBI Synonym Full Names: | adenomatous polyposis coli |
| NCBI Official Symbol: | APC |
| NCBI Official Synonym Symbols: | GS; DP2; DP3; BTPS2; DP2.5; PPP1R46 |
| NCBI Protein Information: | adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46 |
| UniProt Protein Name: | Adenomatous polyposis coli protein |
| UniProt Synonym Protein Names: | Deleted in polyposis 2.5 |
| Protein Family: | APC-related protein |
| UniProt Gene Name: | APC |
| UniProt Entry Name: | APC_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using APC antibody (CAB2818) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human lung using APC antibody (CAB2818) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using APC antibody (CAB2818) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using APC antibody (CAB2818) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of HT-29 cells using APC antibody (CAB2818) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Related Products
