Anti-AP3B1 Antibody (CAB7019)
- SKU:
- CAB7019
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-AP3B1 Antibody |
| Antibody SKU: | CAB7019 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 895-1094 of human AP3B1 (NP_003655.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SKOV3, SW620, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 895-1094 of human AP3B1 (NP_003655.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FGDK MVSI QITL NNTT DRKI ENIH IGEK KLPI GMKM HVFN PIDS LEPE GSIT VSMG IDFC DSTQ TASF QLCT KDDC FNVN IQPP VGEL LLPV AMSE KDFK KEQG VLTG MNET SAVI IAAP QNFT PSVI FQKV VNVA NVGA VPSG QDNI HRFA AKTV HSGS LMLV TVEL KEGS TAQL IINT EKTV IGSV LLRE LKPV LSQG |
| Gene ID: | 8546 |
| Uniprot: | O00203 |
| Cellular Location: | Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Peripheral membrane protein, clathrin-coated vesicle membrane |
| Calculated MW: | 116kDa/121kDa |
| Observed MW: | 132kDa |
| Synonyms: | AP3B1, ADTB3, ADTB3A, HPS, HPS2, PE |
| Background: | This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | AP3B1: a subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. Mutations in AP3B1 cause defects of various cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes, and are the cause of Hermansky-Pudlak syndrome 2. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Vesicle Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: Golgi apparatus; membrane; lysosomal membrane; clathrin coated vesicle membrane Molecular Function:GTP-dependent protein binding; protein phosphatase binding Biological Process: intracellular protein transport; protein targeting to lysosome; melanosome organization and biogenesis; anterograde synaptic vesicle transport; antigen processing and presentation, exogenous lipid antigen via MHC class Ib; positive regulation of NK T cell differentiation; blood coagulation; anterograde axon cargo transport Disease: Hermansky-pudlak Syndrome 2 |
| NCBI Summary: | This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012] |
| UniProt Code: | O00203 |
| NCBI GenInfo Identifier: | 254763431 |
| NCBI Gene ID: | 8546 |
| NCBI Accession: | O00203.3 |
| UniProt Secondary Accession: | O00203,O00580, Q7Z393, Q9HD66, E5RJ68, |
| UniProt Related Accession: | O00203 |
| Molecular Weight: | 116,195 Da |
| NCBI Full Name: | AP-3 complex subunit beta-1 |
| NCBI Synonym Full Names: | adaptor-related protein complex 3, beta 1 subunit |
| NCBI Official Symbol: | AP3B1 |
| NCBI Official Synonym Symbols: | PE; HPS; HPS2; ADTB3; ADTB3A |
| NCBI Protein Information: | AP-3 complex subunit beta-1; beta-3A-adaptin; AP-3 complex beta-3A subunit; adaptor protein complex AP-3 subunit beta-1; adaptor-related protein complex 3 subunit beta-1; clathrin assembly protein complex 3 beta-1 large chain |
| UniProt Protein Name: | AP-3 complex subunit beta-1 |
| UniProt Synonym Protein Names: | Adaptor protein complex AP-3 subunit beta-1; Adaptor-related protein complex 3 subunit beta-1; Beta-3A-adaptin; Clathrin assembly protein complex 3 beta-1 large chain |
| Protein Family: | AP-3 complex |
| UniProt Gene Name: | AP3B1 |
| UniProt Entry Name: | AP3B1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AP3B1 antibody (CAB7019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
Anti-CHD1L Antibody (CAB17558)
