Anti-ANO1 Antibody (CAB10498)
- SKU:
- CAB10498
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-ANO1 Antibody |
| Antibody SKU: | CAB10498 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 628-731 of human ANO1 (NP_060513.5). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HCT116, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 628-731 of human ANO1 (NP_060513.5). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FKGR FVGR PGDY VYIF RSFR MEEC APGG CLME LCIQ LSII MLGK QLIQ NNLF EIGI PKMK KLIR YLKL KQQS PPDH EECV KRKQ RYEV DYNL EPFA GLTP EYME |
| Gene ID: | 55107 |
| Uniprot: | Q5XXA6 |
| Cellular Location: | Cell membrane, Cytoplasm, Multi-pass membrane protein |
| Calculated MW: | 74kDa/97kDa/114kDa |
| Observed MW: | 150kDa |
| Synonyms: | ANO1, DOG1, ORAOV2, TAOS2, TMEM16A |
| Background: |
| UniProt Protein Function: | ANO1: Acts as a calcium-activated chloride channel. Required for normal tracheal development. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Transporter, ion channel; Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane Molecular Function:protein binding; protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity; calcium activated cation channel activity Biological Process: regulation of membrane potential; multicellular organismal development; chloride transport; transmembrane transport; cation transport |
| UniProt Code: | Q5XXA6 |
| NCBI GenInfo Identifier: | 74708278 |
| NCBI Gene ID: | 55107 |
| NCBI Accession: | Q5XXA6.1 |
| UniProt Secondary Accession: | Q5XXA6,Q8IYY8, Q8N7V3, A8KAM3, |
| UniProt Related Accession: | Q5XXA6 |
| Molecular Weight: | |
| NCBI Full Name: | Anoctamin-1 |
| NCBI Synonym Full Names: | anoctamin 1, calcium activated chloride channel |
| NCBI Official Symbol: | ANO1 |
| NCBI Official Synonym Symbols: | DOG1; TAOS2; ORAOV2; TMEM16A |
| NCBI Protein Information: | anoctamin-1; Ca2+-activated Cl- channel; oral cancer overexpressed 2; tumor-amplified and overexpressed sequence 2; discovered on gastrointestinal stromal tumors protein 1; transmembrane protein 16A (eight membrane-spanning domains) |
| UniProt Protein Name: | Anoctamin-1 |
| UniProt Synonym Protein Names: | Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2 |
| Protein Family: | Anoctamin |
| UniProt Gene Name: | ANO1 |
| UniProt Entry Name: | ANO1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ANO1 antibody (CAB10498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U2OS cells using ANO1 antibody (CAB10498) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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