Anti-ANKHD1 Antibody (CAB8224)
- SKU:
- CAB8224
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | ANKHD1 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8224 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1620-1800 of human ANKHD1 (NP_060217.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1620-1800 of human ANKHD1 (NP_060217.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VNSS KYPS LLLH SQEE KTST ATSK TQTR LEGE VTPN SLST SYKT VSLP LSSP NIKL NLTS PKRG QKRE EGWK EVVR RSKK LSVP ASVV SRIM GRGG CNIT AIQD VTGA HIDV DKQK DKNG ERMI TIRG GTES TRYA VQLI NALI QDPA KELE DLIP KNHI RTPA STKS IHAN FSSG VGTT A |
| Gene ID: | 54882 |
| Uniprot: | Q8IWZ3 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 59kDa/63kDa/64kDa/269kDa/277kDa |
| Observed MW: | 269kDa |
| Synonyms: | ANKHD1, MASK, MASK1, PP2500, VBARP |
| Background: | This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. |
| UniProt Protein Function: | ANKHD1: May play a role as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. Isoform 2 may possess an antiapoptotic effect and protect cells during normal cell survival through its regulation of caspases. Belongs to the mask family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA-binding; Apoptosis Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: cytoplasm Molecular Function:protein binding |
| NCBI Summary: | This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq, Sep 2010] |
| UniProt Code: | Q8IWZ3 |
| NCBI GenInfo Identifier: | 74750718 |
| NCBI Gene ID: | 54882 |
| NCBI Accession: | Q8IWZ3.1 |
| UniProt Secondary Accession: | Q8IWZ3,Q149P2, Q8IWZ2, Q8WY90, Q96G77, Q96GK0, Q9H2U0 Q9HA95, Q9NWG4, Q9UPR7, A6NH85, |
| UniProt Related Accession: | Q8IWZ3 |
| Molecular Weight: | 277,175 Da |
| NCBI Full Name: | Ankyrin repeat and KH domain-containing protein 1 |
| NCBI Synonym Full Names: | ankyrin repeat and KH domain containing 1 |
| NCBI Official Symbol: | ANKHD1 |
| NCBI Official Synonym Symbols: | MASK; VBARP; PP2500 |
| NCBI Protein Information: | ankyrin repeat and KH domain-containing protein 1; HIV-1 Vpr-binding ankyrin repeat protein; multiple ankyrin repeats, single KH-domain homolog |
| UniProt Protein Name: | Ankyrin repeat and KH domain-containing protein 1 |
| UniProt Synonym Protein Names: | HIV-1 Vpr-binding ankyrin repeat protein; Multiple ankyrin repeats single KH domain; hMASK |
| Protein Family: | Ankyrin repeat and KH domain-containing protein |
| UniProt Gene Name: | ANKHD1 |
| UniProt Entry Name: | ANKH1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ANKHD1 antibody (CAB8224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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