Anti-ANAPC11 Antibody (CAB15449)
- SKU:
- CAB15449
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-ANAPC11 Antibody |
| Antibody SKU: | CAB15449 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human ANAPC11 (NP_001002245.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human ANAPC11 (NP_001002245.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKVK IKCW NGVA TWLW VAND ENCG ICRM AFNG CCPD CKVP GDDC PLVW GQCS HCFH MHCI LKWL HAQQ VQQH CPMC RQEW KFKE |
| Gene ID: | 51529 |
| Uniprot: | Q9NYG5 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 9kDa/20kDa |
| Observed MW: | 12kDa |
| Synonyms: | ANAPC11, APC11, Apc11p, HSPC214 |
| Background: |
| UniProt Protein Function: | APC11: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex. Belongs to the RING-box family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; Ligase; Cell cycle regulation; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: anaphase-promoting complex; cytosol; nucleoplasm Molecular Function:ubiquitin-protein ligase activity Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of mitotic metaphase/anaphase transition; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle |
| UniProt Code: | Q9NYG5 |
| NCBI GenInfo Identifier: | 19924286 |
| NCBI Gene ID: | 51529 |
| NCBI Accession: | Q9NYG5.1 |
| UniProt Secondary Accession: | Q9NYG5,Q502X9, Q9BW64, Q9P0R2, A8MTT2, B7ZW64, |
| UniProt Related Accession: | Q9NYG5 |
| Molecular Weight: | 9841 Da |
| NCBI Full Name: | Anaphase-promoting complex subunit 11 |
| NCBI Synonym Full Names: | anaphase promoting complex subunit 11 |
| NCBI Official Symbol: | ANAPC11 |
| NCBI Official Synonym Symbols: | APC11; Apc11p; HSPC214 |
| NCBI Protein Information: | anaphase-promoting complex subunit 11 |
| UniProt Protein Name: | Anaphase-promoting complex subunit 11 |
| UniProt Synonym Protein Names: | Cyclosome subunit 11; Hepatocellular carcinoma-associated RING finger protein |
| UniProt Gene Name: | ANAPC11 |
| UniProt Entry Name: | APC11_HUMAN |
 | Western blot analysis of extracts of mouse heart, using ANAPC11 antibody (CAB15449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 120s. |
 | Immunofluorescence analysis of A-549 cells using ANAPC11 antibody (CAB15449) at dilution of 1:300. Blue: DAPI for nuclear staining. |
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