Anti-AMY2A 항체 (CAB8045)

Antibody Name:	AMY2A Rabbit Polyclonal Antibody
Antibody SKU:	CAB8045
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 242-511 of human AMY2A (NP_000690.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse intestine, Rat intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 242-511 of human AMY2A (NP_000690.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KPFI YQEV IDLG GEPI KSSD YFGN GRVT EFKY GAKL GTVI RKWN GEKM SYLK NWGE GWGF VPSD RALV FVDN HDNQ RGHG AGGA SILT FWDA RLYK MAVG FMLA HPYG FTRV MSSY RWPR QFQN GNDV NDWV GPPN NNGV IKEV TINP DTTC GNDW VCEH RWRQ IRNM VIFR NVVD GQPF TNWY DNGS NQVA FGRG NRGF IVFN NDDW SFSL TLQT GLPA GTYC DVIS GDKI NGNC TGIK IYVS DDGK AHFS ISNS AEDP FIAI HAES KL
Gene ID:	279
Uniprot:	P04746
Cellular Location:	Secreted, extracellular space
Calculated MW:	30kDa/57kDa
Observed MW:	58kDa

Synonyms:

AMY2A, AMY2, PA

Background:

This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1, 4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas.

UniProt Protein Function:	AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Secreted; Hydrolase; EC 3.2.1.1; Secreted, signal peptide; Carbohydrate Metabolism - starch and sucrose Chromosomal Location of Human Ortholog: 1p21 Cellular Component: extracellular space; extracellular region Molecular Function:alpha-amylase activity; calcium ion binding; chloride ion binding Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis
NCBI Summary:	This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015]
UniProt Code:	P04746
NCBI GenInfo Identifier:	113803
NCBI Gene ID:	279
NCBI Accession:	P04746.2
UniProt Secondary Accession:	P04746,Q9UBH3, B9EJG1,
UniProt Related Accession:	P04746
Molecular Weight:	511
NCBI Full Name:	Pancreatic alpha-amylase
NCBI Synonym Full Names:	amylase, alpha 2A (pancreatic)
NCBI Official Symbol:	AMY2A
NCBI Official Synonym Symbols:	PA; AMY2
NCBI Protein Information:	pancreatic alpha-amylase; glycogenase; pancreatic amylase alpha 2A; 1,4-alpha-D-glucan glucanohydrolase
UniProt Protein Name:	Pancreatic alpha-amylase
UniProt Synonym Protein Names:	1,4-alpha-D-glucan glucanohydrolase
UniProt Gene Name:	AMY2A
UniProt Entry Name:	AMYP_HUMAN

	Western blot analysis of extracts of various cell lines, using AMY2A antibody (CAB8045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using AMY2A antibody (CAB8045) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using AMY2A antibody (CAB8045) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
AMY2A Antibody (PACO07722)	Human AMY2 (Amylase Alpha 2, Pancreatic) CLIA Kit (HUES01171)
	Human Pancreatic alpha-amylase (AMY2A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)