Anti-AMY1A Antibody (CAB6867)

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SKU:
CAB6867
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Frequently bought together:

Description

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Antibody Name:Anti-AMY1A Antibody
Antibody SKU:CAB6867
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 16-200 of human AMY1A (NP_004029.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, Mouse liver, Mouse pancreas, Mouse heart, Rat liver, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 16-200 of human AMY1A (NP_004029.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QYSS NTQQ GRTS IVHL FEWR WVDI ALEC ERYL APKG FGGV QVSP PNEN VAIH NPFR PWWE RYQP VSYK LCTR SGNE DEFR NMVT RCNN VGVR IYVD AVIN HMCG NAVS AGTS STCG SYFN PGSR DFPA VPYS GWDF NDGK CKTG SGDI ENYN DATQ VRDC RLSG LLDL ALGK DYVR SKIA EYMN H
Gene ID:276
Uniprot:P04745
Cellular Location:Secreted
Calculated MW:57kDa
Observed MW:48kDa
Synonyms:AMY1A, AMY1
Background:Amylases are secreted proteins that hydrolyze 1, 4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein.
UniProt Protein Function:AMY1: Monomer. Belongs to the glycosyl hydrolase 13 family.
UniProt Protein Details:

Protein type:Hydrolase; Carbohydrate Metabolism - starch and sucrose; Secreted; EC 3.2.1.1; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space

Molecular Function:alpha-amylase activity; protein binding; metal ion binding

Biological Process: carbohydrate metabolic process; digestion

NCBI Summary:Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. [provided by RefSeq, Jul 2008]
UniProt Code:P04745
NCBI GenInfo Identifier:1351933
NCBI Gene ID:277
NCBI Accession:P04745.2
UniProt Secondary Accession:P04745,Q13763, Q5T083, A6NJS5, A8K8H6,
UniProt Related Accession:P04745
Molecular Weight:
NCBI Full Name:Alpha-amylase 1
NCBI Synonym Full Names:amylase, alpha 1B (salivary)
NCBI Official Symbol:AMY1B  
NCBI Official Synonym Symbols:AMY1  
NCBI Protein Information:alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1B; amylase, salivary, alpha-1B; 1,4-alpha-D-glucan glucanohydrolase 1
UniProt Protein Name:Alpha-amylase 1
UniProt Synonym Protein Names:1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase
UniProt Gene Name:AMY1A  
UniProt Entry Name:AMY1_HUMAN
Anti-AMY1A Antibody (CAB6867)Western blot analysis of extracts of various cell lines, using AMY1A antibody (CAB6867) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

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