Anti-AMPH Antibody (CAB5389)
- SKU:
- CAB5389
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-AMPH Antibody |
| Antibody SKU: | CAB5389 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-620 of human AMPH (NP_001626.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-620 of human AMPH (NP_001626.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NEVP EVKK EETL LDLD FDPF KPEV TPAG SAGV THSP MSQT LPWD LWTT STDL VQPA SGGS FNGF TQPQ DTSL FTMQ TDQS MICN LAES EQAP PTEP KAEE PLAA VTPA VGLD LGMD TRAE EPVE EAVI IPGA DADA AVGT LVSA AEGA PGEE AEAE KATV PAGE GVSL EEAK IGTE TTEG AESA QPEA EELE ATVP QEKV IPSV VIEP ASNH EEEG ENEI TIGA EPKE TTED AAPP GPTS ETPE LATE QKPI QDPQ PTPS APAM GAAD QLAS AREA SQEL P |
| Gene ID: | 273 |
| Uniprot: | P49418 |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Peripheral membrane protein, cytoskeleton, secretory vesicle, synaptic vesicle membrane |
| Calculated MW: | 71kDa/76kDa |
| Observed MW: | 125KDa |
| Synonyms: | AMPH, AMPH1 |
| Background: | This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11. |
| UniProt Protein Function: | amphiphysin: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 7p14-p13 Cellular Component: synaptic vesicle; synaptic vesicle membrane; cell junction; actin cytoskeleton Molecular Function:protein binding; phospholipid binding Biological Process: synaptic transmission; synaptic vesicle endocytosis; endocytosis; learning |
| NCBI Summary: | This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010] |
| UniProt Code: | P49418 |
| NCBI GenInfo Identifier: | 1351924 |
| NCBI Gene ID: | 273 |
| NCBI Accession: | P49418.1 |
| UniProt Secondary Accession: | P49418,O43538, Q75MJ8, Q75MK5, Q75MM3, Q8N4G0, A4D1X8 A4D1X9, |
| UniProt Related Accession: | P49418 |
| Molecular Weight: | 695 |
| NCBI Full Name: | Amphiphysin |
| NCBI Synonym Full Names: | amphiphysin |
| NCBI Official Symbol: | AMPH |
| NCBI Official Synonym Symbols: | AMPH1 |
| NCBI Protein Information: | amphiphysin; amphiphysin I; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen) |
| UniProt Protein Name: | Amphiphysin |
| Protein Family: | Amphinase |
| UniProt Gene Name: | AMPH |
| UniProt Entry Name: | AMPH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AMPH Polyclonal Antibody (CAB5389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U-251 MG cells using AMPH Rabbit pAb (CAB5389) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Related Products
Human AMPH / Anti-Amphiphysin ELISA Kit
