Anti-AMOTL2 Antibody (CAB16723)
- SKU:
- CAB16723
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-AMOTL2 Antibody |
| Antibody SKU: | CAB16723 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 638-837 of human AMOTL2 (NP_001265612.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, U-251MG, HepG2, Mouse liver, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 638-837 of human AMOTL2 (NP_001265612.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RQFA MDAA ATAA AQRD TTLI RHSP QPSP SSSF NEGL LTGG HRHQ EMES RLKV LHAQ ILEK DAVI KVLQ QRSR RDPG KAIQ GSLR PAKS VPSV FAAA AAGT QGWQ GLSS SERQ TADA PARL TTDR APTE EPVV TAPP AAHA KHGS RDGS TQTE GPPD STST CLPP EPDS LLGC SSSQ RAAS LDSV ATSR VQDL SDMV EILI |
| Gene ID: | 51421 |
| Uniprot: | Q9Y2J4 |
| Cellular Location: | |
| Calculated MW: | 60kDa/86kDa |
| Observed MW: | 60kDa/115kDa |
| Synonyms: | AMOTL2, LCCP |
| Background: | Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene. |
| UniProt Protein Function: | AMOTL2: Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC. Belongs to the angiomotin family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion Chromosomal Location of Human Ortholog: 3q21-q22 Cellular Component: cytosol Molecular Function:protein binding |
| NCBI Summary: | Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9Y2J4 |
| NCBI GenInfo Identifier: | 308153633 |
| NCBI Gene ID: | 51421 |
| NCBI Accession: | Q9Y2J4.3 |
| UniProt Secondary Accession: | Q9Y2J4,Q53EP1, Q96F99, Q9UKB4, A8K6F1, B7Z5Q1, E9PHW3 |
| UniProt Related Accession: | Q9Y2J4 |
| Molecular Weight: | 91,817 Da |
| NCBI Full Name: | Angiomotin-like protein 2 |
| NCBI Synonym Full Names: | angiomotin like 2 |
| NCBI Official Symbol: | AMOTL2 |
| NCBI Official Synonym Symbols: | LCCP |
| NCBI Protein Information: | angiomotin-like protein 2 |
| UniProt Protein Name: | Angiomotin-like protein 2 |
| UniProt Synonym Protein Names: | Leman coiled-coil protein; LCCP |
| Protein Family: | Angiomotin-like protein |
| UniProt Gene Name: | AMOTL2 |
| UniProt Entry Name: | AMOL2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AMOTL2 antibody (CAB16723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using AMOTL2 antibody (CAB16723) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using AMOTL2 antibody (CAB16723) at dilution of 1:200 (40x lens). |
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