Anti-AMFR Antibody (CAB3731)
- SKU:
- CAB3731
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-AMFR Antibody |
| Antibody SKU: | CAB3731 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 384-643 of human AMFR (NP_001135.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | SKOV3, DU145 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 384-643 of human AMFR (NP_001135.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IADN NRVR EEHQ GENL DENL VPVA AAEG RPRL NQHN HFFH FDGS RIAS WLPS FSVE VMHT TNIL GITQ ASNS QLNA MAHQ IQEM FPQV PYHL VLQD LQLT RSVE ITTD NILE GRIQ VPFP TQRS DSIR PALN SPVE RPSS DQEE GETS AQTE RVPL DLSP RLEE TLDF GEVE VEPS EVED FEAR GSRF SKSA DERQ RMLV QRKD ELLQ QARK RFLN KSSE DDAA SESF LPSE GASS DPVT LRRR MLAA AAER RLQK QQTS |
| Gene ID: | 267 |
| Uniprot: | Q9UKV5 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 72kDa |
| Observed MW: | 72kDa |
| Synonyms: | AMFR, GP78, RNF45 |
| Background: | This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. |
| UniProt Protein Function: | AMFR: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis. Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation. Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ligase; Membrane protein, integral; EC 6.3.2.19; Endoplasmic reticulum; Ubiquitin conjugating system; Motility/polarity/chemotaxis; EC 6.3.2.-; Membrane protein, multi-pass; Receptor, misc. Chromosomal Location of Human Ortholog: 16q21 Cellular Component: endoplasmic reticulum membrane; growth cone; protein complex; cell soma; membrane; perinuclear region of cytoplasm; dendrite; integral to membrane; integral to endoplasmic reticulum membrane; nucleus Molecular Function:protein binding; zinc ion binding; ubiquitin-protein ligase activity; receptor activity; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; ER-associated protein catabolic process; protein polyubiquitination; learning and/or memory; unfolded protein response; cell motility; signal transduction; protein oligomerization; aging |
| NCBI Summary: | This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q9UKV5 |
| NCBI GenInfo Identifier: | 113707 |
| NCBI Gene ID: | 267 |
| NCBI Accession: | P26442.1 |
| UniProt Secondary Accession: | Q9UKV5,P26442, Q8IZ70, |
| UniProt Related Accession: | Q9UKV5 |
| Molecular Weight: | 323 |
| NCBI Full Name: | |
| NCBI Synonym Full Names: | autocrine motility factor receptor |
| NCBI Official Symbol: | AMFR |
| NCBI Official Synonym Symbols: | GP78; RNF45 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase AMFR |
| UniProt Protein Name: | E3 ubiquitin-protein ligase AMFR |
| UniProt Synonym Protein Names: | Autocrine motility factor receptor; AMF receptor; RING finger protein 45; gp78 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | AMFR |
| UniProt Entry Name: | AMFR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AMFR antibody (CAB3731) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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