Anti-ALYREF Antibody (CAB6010)

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SKU:
CAB6010
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Frequently bought together:

Description

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Antibody Name:Anti-ALYREF Antibody
Antibody SKU:CAB6010
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human ALYREF
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A-549, B cells, SKOV3, HL-60, Mouse testis, Mouse spleen, Mouse thymus, Rat brain
Immunogen:Recombinant protein of human ALYREF
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10189
Uniprot:Q86V81
Cellular Location:Cytoplasm, Nucleus, Nucleus speckle
Calculated MW:26kDa
Observed MW:30kDa
Synonyms:ALYREF, ALY, ALY/REF, BEF, REF, THOC4
Background:The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
UniProt Protein Function:Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.
NCBI Summary:The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]
UniProt Code:Q86V81
NCBI GenInfo Identifier:48429165
NCBI Gene ID:10189
NCBI Accession:Q86V81.3
UniProt Secondary Accession:Q86V81,O43672,
UniProt Related Accession:Q86V81
Molecular Weight:26 kDa
NCBI Full Name:THO complex subunit 4
NCBI Synonym Full Names:Aly/REF export factor
NCBI Official Symbol:ALYREF  
NCBI Official Synonym Symbols:ALY; BEF; REF; THOC4; ALY/REF  
NCBI Protein Information:THO complex subunit 4
UniProt Protein Name:THO complex subunit 4
UniProt Synonym Protein Names:Ally of AML-1 and LEF-1; Aly/REF export factor; Transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF
Protein Family:Alytesin
UniProt Gene Name:ALYREF  
Anti-ALYREF Antibody (CAB6010)Western blot analysis of extracts of various cell lines, using ALYREF antibody (CAB6010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-ALYREF Antibody (CAB6010)Immunohistochemistry of paraffin-embedded Rat lung using ALYREF Rabbit pAb (CAB6010) at dilution of 1:100 (40x lens).
Anti-ALYREF Antibody (CAB6010)Immunohistochemistry of paraffin-embedded Mouse liver using ALYREF Rabbit pAb (CAB6010) at dilution of 1:100 (40x lens).
Anti-ALYREF Antibody (CAB6010)Immunofluorescence analysis of C6 cells using ALYREF Polyclonal Antibody (CAB6010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-ALYREF Antibody (CAB6010)Immunofluorescence analysis of NIH-3T3 cells using ALYREF Polyclonal Antibody (CAB6010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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