Anti-alpha smooth muscle actin Antibody (CAB7248)

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SKU:
CAB7248
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-alpha smooth muscle actin Antibody
Antibody SKU:CAB7248
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human alpha smooth muscle actin (NP_001135417.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse spleen, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human alpha smooth muscle actin (NP_001135417.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MCEE EDST ALVC DNGS GLCK AGFA GDDA PRAV FPSI VGRP RHQG VMVG MGQK DSYV GDEA QSKR GILT LKYP IEHG IITN WDDM EKIW HHSF YNEL RVAP EEHP TLLT EAPL NPKA NREK MTQI MFET FNVP AMYV AIQA VLSL YASG RTTG IVLD SGDG VTHN VPIY EGYA LPHA IMRL DLAG RDLT DYLM KILT ERGY SFVT TAER EIVR DIKE KLCY VALD FENE MATA ASSS SLEK SYEL PDGQ VITI GNER FRCP ETLF QPSF IGME SAGI HETT YNSI MKCD IDIR KDLY ANNV LSGG TTMY PGIA DRMQ KEIT ALAP STMK IKII APPE RKYS VWIG GSIL ASLS TFQQ MWIS KQEY DEAG PSIV HRKC F
Gene ID:59
Uniprot:P62736
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:42kDa
Observed MW:42kDa
Synonyms:AAT6, ACTSA, MYMY5, ACTA2, actin
Background:The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
UniProt Protein Function:ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.
UniProt Protein Details:

Protein type:Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton

Molecular Function:protein kinase binding; ATP binding

Biological Process: muscle contraction; response to virus; regulation of blood pressure; vascular smooth muscle contraction

Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6

NCBI Summary:The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
UniProt Code:P62736
NCBI GenInfo Identifier:51316972
NCBI Gene ID:59
NCBI Accession:P62736.1
UniProt Secondary Accession:P62736,P03996, P04108, Q6FI19, B2R8A4,
UniProt Related Accession:P62736
Molecular Weight:42,009 Da
NCBI Full Name:Actin, aortic smooth muscle
NCBI Synonym Full Names:actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol:ACTA2  
NCBI Official Synonym Symbols:AAT6; ACTSA; MYMY5  
NCBI Protein Information:actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
UniProt Protein Name:Actin, aortic smooth muscle
UniProt Synonym Protein Names:Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Protein Family:Actin
UniProt Gene Name:ACTA2  
UniProt Entry Name:ACTA_HUMAN
Anti-alpha smooth muscle actin Antibody (CAB7248)Western blot analysis of extracts of various cell lines, using alpha smooth muscle actin antibody (CAB7248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Anti-alpha smooth muscle actin Antibody (CAB7248)Immunohistochemistry of paraffin-embedded Human placenta using alpha smooth muscle actin antibody (CAB7248) at dilution of 1:100 (40x lens).
Anti-alpha smooth muscle actin Antibody (CAB7248)Immunohistochemistry of paraffin-embedded Rat lung using alpha smooth muscle actin antibody (CAB7248) at dilution of 1:100 (40x lens).
Anti-alpha smooth muscle actin Antibody (CAB7248)Confocal immunofluorescence analysis of human colon carcinoma using alpha smooth muscle actin Polyclonal Antibody (CAB7248) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-alpha smooth muscle actin Antibody (CAB7248)Confocal immunofluorescence analysis of mouse colon using alpha smooth muscle actin Polyclonal Antibody (CAB7248) at dilution of 1:100. Blue: DAPI for nuclear staining.

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