Anti-alpha smooth muscle actin Antibody (CAB1011)
- SKU:
- CAB1011
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-alpha smooth muscle actin Antibody |
| Antibody SKU: | CAB1011 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human alpha smooth muscle actin (NP_001135417.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | RD, Mouse brain, Mouse large intestine, Rat stomach, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human alpha smooth muscle actin (NP_001135417.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MCEE EDST ALVC DNGS GLCK AGFA GDDA PRAV FPSI VGRP RHQG VMVG MGQK DSYV GDEA QSKR GILT LKYP IEHG IITN WDDM EKIW HHSF YNEL RVAP EEHP TLLT EAPL NPKA NREK MTQI |
| Gene ID: | 59 |
| Uniprot: | P62736 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 42kDa |
| Observed MW: | 42kDa |
| Synonyms: | AAT6, ACTSA, MYMY5, ACTA2, actin |
| Background: | The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| UniProt Protein Function: | ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family. |
| UniProt Protein Details: | Protein type:Contractile; Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10q23.3 Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton Molecular Function:protein kinase binding; ATP binding Biological Process: muscle contraction; response to virus; regulation of blood pressure; vascular smooth muscle contraction Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6 |
| NCBI Summary: | The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| UniProt Code: | P62736 |
| NCBI GenInfo Identifier: | 51316972 |
| NCBI Gene ID: | 59 |
| NCBI Accession: | P62736.1 |
| UniProt Secondary Accession: | P62736,P03996, P04108, Q6FI19, B2R8A4, |
| UniProt Related Accession: | P62736 |
| Molecular Weight: | 42,009 Da |
| NCBI Full Name: | Actin, aortic smooth muscle |
| NCBI Synonym Full Names: | actin, alpha 2, smooth muscle, aorta |
| NCBI Official Symbol: | ACTA2 |
| NCBI Official Synonym Symbols: | AAT6; ACTSA; MYMY5 |
| NCBI Protein Information: | actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein |
| UniProt Protein Name: | Actin, aortic smooth muscle |
| UniProt Synonym Protein Names: | Alpha-actin-2; Cell growth-inhibiting gene 46 protein |
| Protein Family: | Actin |
| UniProt Gene Name: | ACTA2 |
| UniProt Entry Name: | ACTA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using alpha smooth muscle actin antibody (CAB1011) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human placenta using alpha smooth muscle actin antibody (CAB1011) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat ovary using alpha smooth muscle actin antibody (CAB1011) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using alpha smooth muscle actin antibody (CAB1011) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat rectum using alpha smooth muscle actin Rabbit pAb (CAB1011) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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