Anti-ALOX15B Antibody (CAB6865)
- SKU:
- CAB6865
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ALOX15B Antibody |
| Antibody SKU: | CAB6865 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALOX15B (NP_001132.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HL-60, Mouse ovary |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALOX15B (NP_001132.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEF RVRV STGE AFGA GTWD KVSV SIVG TRGE SPPL PLDN LGKE FTAG AEED FQVT LPED VGRV LLLR VHKA PPVL PLLG PLAP DAWF CRWF QLTP PRGG HLLF PCYQ WLEG AGTL VLQE GTAK VSWA DHHP VLQQ QRQE ELQA RQEM YQWK AYNP GWPH CLDE KTVE DLEL NIKY STAK NANF YLQA GSAF AEMK IKGL |
| Gene ID: | 247 |
| Uniprot: | O15296 |
| Cellular Location: | Cell membrane, Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, cytoskeleton, cytosol |
| Calculated MW: | 67kDa/69kDa/72kDa/75kDa |
| Observed MW: | 76kDa |
| Synonyms: | ALOX15B, 15-LOX-2 |
| Background: | This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; EC 1.13.11.33; Apoptosis; Lipid Metabolism - arachidonic acid; Cell cycle regulation; Oxidoreductase Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: cytoplasm; cytoskeleton; cytosol; extrinsic to membrane; intracellular; membrane; plasma membrane Molecular Function:arachidonate 15-lipoxygenase activity; calcium ion binding; iron ion binding; lipoxygenase activity Biological Process: apoptosis; arachidonic acid metabolic process; hepoxilin biosynthetic process; lipid metabolic process; lipoxygenase pathway; negative regulation of cell cycle; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of growth |
| NCBI Summary: | This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15296 |
| NCBI GenInfo Identifier: | 317373425 |
| NCBI Gene ID: | 247 |
| NCBI Accession: | O15296.3 |
| UniProt Secondary Accession: | O15296,Q8IYQ2, Q8TEV3, Q8TEV4, Q8TEV5, Q8TEV6, Q9UKM4 D3DTR2, |
| UniProt Related Accession: | O15296 |
| Molecular Weight: | 72,523 Da |
| NCBI Full Name: | Arachidonate 15-lipoxygenase B |
| NCBI Synonym Full Names: | arachidonate 15-lipoxygenase, type B |
| NCBI Official Symbol: | ALOX15B |
| NCBI Official Synonym Symbols: | 15-LOX-2 |
| NCBI Protein Information: | arachidonate 15-lipoxygenase B |
| UniProt Protein Name: | Arachidonate 15-lipoxygenase B |
| UniProt Synonym Protein Names: | 15-lipoxygenase 2; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb (EC:1.13.11.-) |
| Protein Family: | Arachidonate 15-lipoxygenase |
| UniProt Gene Name: | ALOX15B |
| UniProt Entry Name: | LX15B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ALOX15B antibody (CAB6865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of MCF7 cells using ALOX15B antibody (CAB6865). |