Anti-ALG2 항체 (CAB7843)

Antibody Name:	Anti-ALG2 Antibody
Antibody SKU:	CAB7843
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 197-416 of human ALG2 (NP_149078.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 197-416 of human ALG2 (NP_149078.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DVLY PSLN VTSF DSVV PEKL DDLV PKGK KFLL LSIN RYER KKNL TLAL EALV QLRG RLTS QDWE RVHL IVAG GYDE RVLE NVEH YQEL KKMV QQSD LGQY VTFL RSFS DKQK ISLL HSCT CVLY TPSN EHFG IVPL EAMY MQCP VIAV NSGG PLES IDHS VTGF LCEP DPVH FSEA IEKF IREP SLKA TMGL AGRA RVKE KFSP EAFT EQLY RYVT KLLV
Gene ID:	85365
Uniprot:	Q9H553
Cellular Location:	Membrane, Single-pass membrane protein
Calculated MW:	37kDa/47kDa
Observed MW:	47kDa

Synonyms:

ALG2, CDG1I, CDGIi, CMS14, CMSTA3, NET38, hALPG2, alpha-1, 3/1

Background:

This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1, 3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ALG2: Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)- dolichol diphosphate. Defects in ALG2 are the cause of congenital disorder of glycosylation type 1I (CDG1I). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.4.1.257; Transferase; Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.132 Chromosomal Location of Human Ortholog: 9q22.33 Cellular Component: cytoplasm; endoplasmic reticulum membrane; membrane; nucleus; perinuclear region of cytoplasm Molecular Function:alpha-1,3-mannosyltransferase activity; calcium-dependent protein binding; protein binding; protein heterodimerization activity; protein N-terminus binding Biological Process: dolichol-linked oligosaccharide biosynthetic process; protein amino acid glycosylation in endoplasmic reticulum; response to calcium ion Disease: Congenital Disorder Of Glycosylation, Type Ii; Myasthenic Syndrome, Congenital, With Tubular Aggregates 3
NCBI Summary:	This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]
UniProt Code:	Q9H553
NCBI GenInfo Identifier:	46395991
NCBI Gene ID:	85365
NCBI Accession:	Q9H553.1
UniProt Secondary Accession:	Q9H553,Q8NBX2, Q8NC39, A2A2Y0,
UniProt Related Accession:	Q9H553
Molecular Weight:	37,017 Da
NCBI Full Name:	Alpha-1,3/1,6-mannosyltransferase ALG2
NCBI Synonym Full Names:	ALG2, alpha-1,3/1,6-mannosyltransferase
NCBI Official Symbol:	ALG2
NCBI Official Synonym Symbols:	CDGIi; CMS14; NET38; CMSTA3; hALPG2
NCBI Protein Information:	alpha-1,3/1,6-mannosyltransferase ALG2
UniProt Protein Name:	Alpha-1,3/1,6-mannosyltransferase ALG2
UniProt Synonym Protein Names:	Asparagine-linked glycosylation protein 2 homolog; GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase; GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Protein Family:	Alpha-1,3/1,6-mannosyltransferase
UniProt Gene Name:	ALG2
UniProt Entry Name:	ALG2_HUMAN

	Western blot analysis of extracts of various cell lines, using ALG2 antibody (CAB7843) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using ALG2 Antibody (CAB7843) at dilution of 1:100 (40x lens).

Antibodies

Anti-ALG2 Antibody (CAB15203)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)