Anti-AKT3 Antibody (CAB12909)
- SKU:
- CAB12909
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-AKT3 Antibody |
| Antibody SKU: | CAB12909 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 93-154 of human AKT3 (NP_859029.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 93-154 of human AKT3 (NP_859029.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EERE EWTE AIQA VADR LQRQ EEER MNCS PTSQ IDNI GEEE MDAS TTHH KRKT MNDF DYLK LL |
| Gene ID: | 10000 |
| Uniprot: | Q9Y243 |
| Cellular Location: | Cytoplasm, Membrane, Nucleus, Peripheral membrane protein |
| Calculated MW: | 54kDa/55kDa |
| Observed MW: | 60kDa |
| Synonyms: | AKT3, MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2 |
| Background: | The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. |
| UniProt Protein Function: | Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.1; AGC group; AKT family Chromosomal Location of Human Ortholog: 1q44 Cellular Component: cytoplasm; Golgi apparatus; intracellular; nucleoplasm; plasma membrane Molecular Function:ATP binding; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: mitochondrial genome maintenance; peptidyl-serine phosphorylation; protein amino acid phosphorylation; signal transduction Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2 |
| NCBI Summary: | The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y243 |
| NCBI GenInfo Identifier: | 12643943 |
| NCBI Gene ID: | 10000 |
| NCBI Accession: | Q9Y243.1 |
| UniProt Secondary Accession: | Q9Y243,Q0VAA6, Q5VTI1, Q5VTI2, Q96QV3, Q9UFP5, |
| UniProt Related Accession: | Q9Y243 |
| Molecular Weight: | 54,032 Da |
| NCBI Full Name: | RAC-gamma serine/threonine-protein kinase |
| NCBI Synonym Full Names: | AKT serine/threonine kinase 3 |
| NCBI Official Symbol: | AKT3 |
| NCBI Official Synonym Symbols: | MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma |
| NCBI Protein Information: | RAC-gamma serine/threonine-protein kinase |
| UniProt Protein Name: | RAC-gamma serine/threonine-protein kinase |
| UniProt Synonym Protein Names: | Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2 |
| Protein Family: | RAC-gamma serine/threonine-protein kinase |
| UniProt Gene Name: | AKT3 |
| UniProt Entry Name: | AKT3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AKT3 antibody (CAB12909) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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