Anti-AKR7A2 항체 (CAB1227)

Antibody Name:	Anti-AKR7A2 Antibody
Antibody SKU:	CAB1227
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-359 of human AKR7A2 (NP_003680.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney, Mouse testis, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-359 of human AKR7A2 (NP_003680.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VKIA TKAN PWDG KSLK PDSV RSQL ETSL KRLQ CPQV DLFY LHAP DHGT PVEE TLHA CQRL HQEG KFVE LGLS NYAS WEVA EICT LCKS NGWI LPTV YQGM YNAT TRQV ETEL FPCL RHFG LRFY AYNP LAGG LLTG KYKY EDKD GKQP VGRF FGNS WAET YRNR FWKE HHFE AIAL VEKA LQAA YGAS APSV TSAA LRWM YHHS QLQG AHGD AVIL GMSS LEQL EQNL AATE EGPL EPAV VDAF NQAW HLVA HECP NYFR
Gene ID:	8574
Uniprot:	O43488
Cellular Location:	Cytoplasm, Golgi apparatus
Calculated MW:	39kDa
Observed MW:	35kDa

Synonyms:

AKR7A2, AFAR, AFAR1, AFB1-AR1, AKR7

Background:

The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	AKR7A2: Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2- nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.1.1.n11 Chromosomal Location of Human Ortholog: 1p36.13 Cellular Component: Golgi apparatus; mitochondrion; cytosol Molecular Function:electron carrier activity; aldehyde reductase activity; phenanthrene-9,10-epoxide hydrolase activity Biological Process: xenobiotic metabolic process; carbohydrate metabolic process; aldehyde metabolic process
NCBI Summary:	The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. [provided by RefSeq, Oct 2011]
UniProt Code:	O43488
NCBI GenInfo Identifier:	38372871
NCBI Gene ID:	8574
NCBI Accession:	O43488.3
UniProt Secondary Accession:	O43488,O75749, Q5TG63,
UniProt Related Accession:	O43488
Molecular Weight:	359
NCBI Full Name:	Aflatoxin B1 aldehyde reductase member 2
NCBI Synonym Full Names:	aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
NCBI Official Symbol:	AKR7A2
NCBI Official Synonym Symbols:	AFAR; AKR7; AFAR1; AFB1-AR1
NCBI Protein Information:	aflatoxin B1 aldehyde reductase member 2; AFB1-AR 1; HEL-S-166mP; SSA reductase; aldoketoreductase 7; AFB1 aldehyde reductase 1; succinic semialdehyde reductase; aflatoxin beta1 aldehyde reductase; epididymis secretory sperm binding protein Li 166mP
UniProt Protein Name:	Aflatoxin B1 aldehyde reductase member 2
UniProt Synonym Protein Names:	AFB1 aldehyde reductase 1; AFB1-AR 1; Aldoketoreductase 7; Succinic semialdehyde reductase; SSA reductase
Protein Family:	Aflatoxin B1 aldehyde reductase
UniProt Gene Name:	AKR7A2
UniProt Entry Name:	ARK72_HUMAN

	Western blot analysis of extracts of various cell lines, using AKR7A2 antibody (CAB1227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunohistochemistry of paraffin-embedded mouse heart using AKR7A2 antibody (CAB1227) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using AKR7A2 antibody (CAB1227). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)