Anti-AGXT Antibody (CAB8397)
- SKU:
- CAB8397
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-AGXT Antibody |
| Antibody SKU: | CAB8397 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 183-392 of human AGXT (NP_000021.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | LO2, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 183-392 of human AGXT (NP_000021.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DSVA SLGG TPLY MDRQ GIDI LYSG SQKA LNAP PGTS LISF SDKA KKKM YSRK TKPF SFYL DIKW LANF WGCD DQPR MYHH TIPV ISLY SLRE SLAL IAEQ GLEN SWRQ HREA AAYL HGRL QALG LQLF VKDP ALRL PTVT TVAV PAGY DWRD IVSY VIDH FDIE IMGG LGPS TGKV LRIG LLGC NATR ENVD RVTE ALRA ALQH CPKK KL |
| Gene ID: | 189 |
| Uniprot: | P21549 |
| Cellular Location: | Mitochondrion, Peroxisome |
| Calculated MW: | 43kDa |
| Observed MW: | 43kDa |
| Synonyms: | AGXT, AGT, AGT1, AGXT1, PH1, SPAT, SPT, TLH6 |
| Background: | This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. |
| UniProt Protein Function: | AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.51; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Motility/polarity/chemotaxis; EC 2.6.1.44 Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: peroxisomal matrix; peroxisome Molecular Function:alanine-glyoxylate transaminase activity; amino acid binding; protein binding; protein homodimerization activity; protein self-association; pyridoxal phosphate binding; receptor binding; serine-pyruvate transaminase activity; transaminase activity Biological Process: glycine biosynthetic process, by transamination of glyoxylate; glyoxylate catabolic process; glyoxylate metabolic process; L-alanine catabolic process; L-cysteine catabolic process; proteasomal protein catabolic process Disease: Hyperoxaluria, Primary, Type I |
| NCBI Summary: | This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P21549 |
| NCBI GenInfo Identifier: | 134855 |
| NCBI Gene ID: | 189 |
| NCBI Accession: | P21549.1 |
| UniProt Secondary Accession: | P21549,Q53QU6, |
| UniProt Related Accession: | P21549 |
| Molecular Weight: | 43010 Da |
| NCBI Full Name: | Serine--pyruvate aminotransferase |
| NCBI Synonym Full Names: | alanine-glyoxylate aminotransferase |
| NCBI Official Symbol: | AGXT |
| NCBI Official Synonym Symbols: | AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1 |
| NCBI Protein Information: | serine--pyruvate aminotransferase |
| UniProt Protein Name: | Serine--pyruvate aminotransferase |
| UniProt Synonym Protein Names: | Alanine--glyoxylate aminotransferase (EC:2.6.1.44); AGT |
| Protein Family: | Serine--pyruvate aminotransferase |
| UniProt Gene Name: | AGXT |
| UniProt Entry Name: | SPYA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AGXT antibody (CAB8397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human liver using AGXT Rabbit pAb (CAB8397) at dilution of 1:150 (40x lens). |
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