Anti-AGRN 항체 (CAB17320)

Antibody Name:	Anti-AGRN Antibody
Antibody SKU:	CAB17320
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-350 of human AGRN (NP_940978.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Mouse brain, Mouse kidney, Rat kidney, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-350 of human AGRN (NP_940978.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TCPE RALE RREE EANV VLTG TVEE ILNV DPVQ HTYS CKVR VWRY LKGK DLVA RESL LDGG NKVV ISGF GDPL ICDN QVST GDTR IFFV NPAP PYLW PAHK NELM LNSS LMRI TLRN LEEV EFCV EDKP GTHF TPVP PTPP DACR GMLC GFGA VCEP NAEG PGRA SCVC KKSP CPSV VAPV CGSD ASTY SNEC ELQR AQCS QQRR IRLL SRGP CGSR DPCS NVTC SFGS TCAR SADG LTAS CLCP ATCR GAPE GTVC GSDG ADYP GECQ LLRR ACAR QENV FKKF DGPC DPCQ GALP DPSR SCRV NPRT RRPE MLLR PESC P
Gene ID:	375790
Uniprot:	O00468
Cellular Location:
Calculated MW:	217kDa
Observed MW:	98kDa/217kDa

Synonyms:

AGRN, AGRIN, CMS8, CMSPPD, agrin

Background:

This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	agrin: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
UniProt Protein Details:	Protein type:Cell adhesion; Cell development/differentiation; Extracellular matrix; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: basal lamina; cell junction; cytosol; extracellular matrix; extracellular region; Golgi lumen; integral component of membrane; lysosomal lumen; plasma membrane; synapse Molecular Function:calcium ion binding; heparan sulfate proteoglycan binding; laminin binding; protein binding; sialic acid binding; structural constituent of cytoskeleton Biological Process: clustering of voltage-gated sodium channels; cytoskeleton organization; extracellular matrix organization; G-protein coupled acetylcholine receptor signaling pathway; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; positive regulation of filopodium formation; positive regulation of GTPase activity; positive regulation of synaptic growth at neuromuscular junction; positive regulation of transcription from RNA polymerase II promoter; receptor clustering; retinoid metabolic process; signal transduction; synapse organization Disease: Myasthenic Syndrome, Congenital, With Pre- And Postsynaptic Defects
NCBI Summary:	This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
UniProt Code:	O00468
NCBI GenInfo Identifier:	54873613
NCBI Gene ID:	375790
NCBI Accession:	NP_940978.2
UniProt Secondary Accession:	O00468,Q5SVA1, Q5SVA2, Q60FE1, Q7KYS8, Q8N4J5, Q96IC1 Q9BTD4,
UniProt Related Accession:	O00468
Molecular Weight:
NCBI Full Name:	agrin isoform 2
NCBI Synonym Full Names:	agrin
NCBI Official Symbol:	AGRN
NCBI Official Synonym Symbols:	CMS8; AGRIN; CMSPPD
NCBI Protein Information:	agrin
UniProt Protein Name:	Agrin
Protein Family:	Agrin
UniProt Gene Name:	AGRN

	Western blot analysis of extracts of various cell lines, using AGRN antibody (CAB17320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using AGRN Rabbit pAb (CAB17320) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using AGRN Rabbit pAb (CAB17320) at dilution of 1:100. Blue: DAPI for nuclear staining.

ELISA Kits

Human Agrin ELISA Kit

Human CAF (c-terminal fragemnte of agrin) ELISA Kit (HUFI04702)

Human AGRN (Agrin) CLIA Kit (HUES00219)

Human AGRN (Agrin) ELISA Kit (HUES01528)

Human Agrin (AGRN) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)