Anti-AGO1 Antibody (CAB6022)
- SKU:
- CAB6022
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-AGO1 Antibody |
| Antibody SKU: | CAB6022 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human AGO1 (NP_036331.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:500 - 1:1000 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-1080, K-562, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human AGO1 (NP_036331.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEAG PSGA AAGA YLPP LQQV FQAP RRPG IGTV GKPI KLLA NYFE VDIP KIDV YHYE VDIK PDKC PRRV NREV VEYM VQHF KPQI FGDR KPVY DGKK NIYT VTAL PIGN ERVD FEVT IPGE GKDR IFKV SIKW LAIV SWRM LHEA LVSG QIPV PLES VQAL DVAM RHLA SMRY TPVG RSFF |
| Gene ID: | 26523 |
| Uniprot: | Q9UL18 |
| Cellular Location: | Cytoplasm, P-body |
| Calculated MW: | 97kDa |
| Observed MW: | 110kDa |
| Synonyms: | AGO1, EIF2C, EIF2C1, GERP95, Q99, hAgo1 |
| Background: | This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. |
| UniProt Code: | Q9UL18 |
| NCBI GenInfo Identifier: | 88984241 |
| NCBI Gene ID: | 26523 |
| NCBI Accession: | Q9UL18.3 |
| UniProt Secondary Accession: | Q9UL18,Q5TA57, Q6P4S0, |
| UniProt Related Accession: | Q9UL18 |
| Molecular Weight: | 857 |
| NCBI Full Name: | Protein argonaute-1 |
| NCBI Synonym Full Names: | argonaute RISC catalytic component 1 |
| NCBI Official Symbol: | AGO1 |
| NCBI Official Synonym Symbols: | Q99; EIF2C; EIF2C1; GERP95 |
| NCBI Protein Information: | protein argonaute-1; hAgo1; eIF2C 1; eIF-2C 1; argonaute1; argonaute 1; putative RNA-binding protein Q99; Golgi Endoplasmic Reticulum protein 95 kDa; eukaryotic translation initiation factor 2C, 1 |
| UniProt Protein Name: | Protein argonaute-1 |
| UniProt Synonym Protein Names: | Argonaute RISC catalytic component 1; Eukaryotic translation initiation factor 2C 1; eIF-2C 1; eIF2C 1; Putative RNA-binding protein Q99 |
| Protein Family: | Protein argonaute |
| UniProt Gene Name: | AGO1 |
| UniProt Entry Name: | AGO1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AGO1 antibody (CAB6022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunoprecipitation analysis of 600ug extracts of Mouse spleen cells using 3ug AGO1 antibody (CAB6022). Western blot was performed from the immunoprecipitate using AGO1 antibody (CAB6022) at a dilition of 1:500. |
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