Anti-ADAM32 Antibody (CAB14977)

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SKU:
CAB14977
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-ADAM32 Antibody
Antibody SKU:CAB14977
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 703-787 of human ADAM32 (NP_659441.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, 293T, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 703-787 of human ADAM32 (NP_659441.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ARKQ LKKW FAKE EEFP SSES KSEG STQT YASQ SSSE GSTQ TYAS QTRS ESSS QADT SKSK SEDS AEAY TSRS KSQD STQT QSSS N
Gene ID:203102
Uniprot:Q8TC27
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:87kDa
Observed MW:88kDa
Synonyms:ADAM32
Background:This gene encodes a member of the disintegrin family of membrane-anchored proteins that play a role in diverse biological processes such as brain development, fertilization, tumor development and inflammation. This gene is predominantly expressed in the testis. The encoded protein undergoes proteolytic processing to generate a mature polypeptide comprised of an metalloprotease, disintegrin and epidermal growth factor-like domains. This gene is located in a cluster of other disintegrin and metallopeptidase family genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:ADAM32: May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p11.22

NCBI Summary:This gene encodes a member of the disintegrin family of membrane-anchored proteins that play a role in diverse biological processes such as brain development, fertilization, tumor development and inflammation. This gene is predominantly expressed in the testis. The encoded protein undergoes proteolytic processing to generate a mature polypeptide comprised of an metalloprotease, disintegrin and epidermal growth factor-like domains. This gene is located in a cluster of other disintegrin and metallopeptidase family genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
UniProt Code:Q8TC27
NCBI GenInfo Identifier:296434389
NCBI Gene ID:203102
NCBI Accession:Q8TC27.2
UniProt Secondary Accession:Q8TC27,Q8TC42,
UniProt Related Accession:Q8TC27
Molecular Weight:87,948 Da
NCBI Full Name:Disintegrin and metalloproteinase domain-containing protein 32
NCBI Synonym Full Names:ADAM metallopeptidase domain 32
NCBI Official Symbol:ADAM32  
NCBI Protein Information:disintegrin and metalloproteinase domain-containing protein 32
UniProt Protein Name:Disintegrin and metalloproteinase domain-containing protein 32
Protein Family:Disintegrin and metalloproteinase domain-containing protein
UniProt Gene Name:ADAM32  
UniProt Entry Name:ADA32_HUMAN
Anti-ADAM32 Antibody (CAB14977)Western blot analysis of extracts of various cell lines, using ADAM32 antibody (CAB14977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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