Anti-ADAM22 항체 (CAB10030)

Antibody Name:	Anti-ADAM22 Antibody
Antibody SKU:	CAB10030
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 223-400 of human ADAM22 (NP_004185.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:4000
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Mouse brain, Mouse kidney, Mouse heart, Rat kidney, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 223-400 of human ADAM22 (NP_004185.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SKRQ LRRY PRNV EEET KYIE LMIV NDHL MFKK HRLS VVHT NTYA KSVV NMAD LIYK DQLK TRIV LVAM ETWA TDNK FAIS ENPL ITLR EFMK YRRD FIKE KSDA VHLF SGSQ FESS RSGA AYIG GICS LLKG GGVN EFGK TDLM AVTL AQSL AHNI GIIS DKRK LASG ECKC EDTW SG
Gene ID:	53616
Uniprot:	Q9P0K1
Cellular Location:	Cell projection, Membrane, Single-pass type I membrane protein, axon
Calculated MW:	91kDa/95kDa/96kDa/100kDa
Observed MW:	100kDa

Synonyms:

ADAM22, ADAM 22, MDC2

Background:

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.

UniProt Protein Function:	Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein (PubMed:19692335). Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.
NCBI Summary:	This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010]
UniProt Code:	Q9P0K1
NCBI GenInfo Identifier:	62204768
NCBI Gene ID:	53616
NCBI Accession:	AAH92465.1
UniProt Related Accession:	Q9P0K1
Molecular Weight:
NCBI Full Name:	ADAM22 protein
NCBI Synonym Full Names:	ADAM metallopeptidase domain 22
NCBI Official Symbol:	ADAM22
NCBI Official Synonym Symbols:	MDC2; EIEE61; ADAM 22
NCBI Protein Information:	disintegrin and metalloproteinase domain-containing protein 22
UniProt Protein Name:	Disintegrin and metalloproteinase domain-containing protein 22
UniProt Synonym Protein Names:	Metalloproteinase-disintegrin ADAM22-3; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
UniProt Gene Name:	ADAM22

Western blot analysis of extracts of various cell lines, using ADAM22 antibody (CAB10030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)