Anti-ACTN2 Antibody (CAB3718)
- SKU:
- CAB3718
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ACTN2 Antibody |
| Antibody SKU: | CAB3718 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACTN2 (NP_001094.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, HT-29, HeLa, Mouse heart, Mouse skeletal muscle, Mouse lung, Rat liver, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACTN2 (NP_001094.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNQI EPGV QYNY VYDE DEYM IQEE EWDR DLLL DPAW EKQQ RKTF TAWC NSHL RKAG TQIE NIEE DFRN GLKL MLLL EVIS GERL PKPD RGKM RFHK IANV NKAL DYIA SKGV KLVS IGAE EIVD GNVK MTLG MIWT IILR FAIQ DISV EETS AKEG LLLW CQRK TAPY RNVN IQNF HTSW KDGL GLCA LIHR HRPD LIDY SKLN KDDP IGNI NLAM EIAE KHLD IPKM LDAE DIVN TPKP DERA IMTY VS |
| Gene ID: | 88 |
| Uniprot: | P35609 |
| Cellular Location: | Cytoplasm, Z line, myofibril, sarcomere |
| Calculated MW: | 103kDa |
| Observed MW: | 104kDa |
| Synonyms: | ACTN2, CMD1AA, CMH23 |
| Background: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 1q42-q43 Cellular Component: cortical actin cytoskeleton; cytoskeleton; focal adhesion; extracellular region; dendritic spine; actin filament; pseudopodium; Z disc; cytosol; filopodium Molecular Function:actin filament binding; protein dimerization activity; integrin binding; identical protein binding; protein binding; LIM domain binding; structural constituent of muscle; cytoskeletal protein binding; titin binding; calcium ion binding; FATZ binding; thyroid hormone receptor coactivator activity Biological Process: focal adhesion formation; platelet activation; negative regulation of potassium ion transport; positive regulation of potassium ion transport; muscle filament sliding; protein homotetramerization; regulation of apoptosis; synaptic transmission; regulation of membrane potential; platelet degranulation; microspike biogenesis; blood coagulation; cell adhesion Disease: Cardiomyopathy, Dilated, 1aa |
| NCBI Summary: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013] |
| UniProt Code: | P35609 |
| NCBI GenInfo Identifier: | 543742 |
| NCBI Gene ID: | 88 |
| NCBI Accession: | P35609.1 |
| UniProt Secondary Accession: | P35609,Q86TF4, Q86TI8, B1ANE4, B2RCS5, |
| UniProt Related Accession: | P35609 |
| Molecular Weight: | 103,920 Da |
| NCBI Full Name: | Alpha-actinin-2 |
| NCBI Synonym Full Names: | actinin, alpha 2 |
| NCBI Official Symbol: | ACTN2 |
| NCBI Official Synonym Symbols: | CMD1AA |
| NCBI Protein Information: | alpha-actinin-2; F-actin cross-linking protein; alpha-actinin skeletal muscle |
| UniProt Protein Name: | Alpha-actinin-2 |
| UniProt Synonym Protein Names: | Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein |
| Protein Family: | Alpha-actinin |
| UniProt Gene Name: | ACTN2 |
| UniProt Entry Name: | ACTN2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACTN2 antibody (CAB3718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells using ACTN2 antibody (CAB3718) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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