Anti-ACSS2 항체 (CAB13596)

Antibody Name:	Anti-ACSS2 Antibody
Antibody SKU:	CAB13596
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 367-606 of human ACSS2 (NP_001229322.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 367-606 of human ACSS2 (NP_001229322.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VDTF WQTE TGGH MLTP LPGA TPMK PGSA TFPF FGVA PAIL NESG EELE GEAE GYLV FKQP WPGI MRTV YGNH ERFE TTYF KKFP GYYV TGDG CQRD QDGY YWIT GRID DMLN VSGH LLST AEVE SALV EHEA VAEA AVVG HPHP VKGE CLYC FVTL CDGH TFSP KLTE ELKK QIRE KIGP IATP DYIQ NAPG LPKT RSGK IMRR VLRK IAQN DHDL GDMS TVAD PSVI SHLF SHRC LTIQ
Gene ID:	55902
Uniprot:	Q9NR19
Cellular Location:	Cytoplasm
Calculated MW:	78kDa/80kDa
Observed MW:	78kDa

Synonyms:

ACSS2, ACAS2, ACECS, ACS, ACSA, dJ1161H23.1

Background:

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ACSS2: Activates acetate so that it can be used for lipid synthesis or for energy generation. Monomer. Belongs to the ATP-dependent AMP-binding enzyme family.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 6.2.1.1; Ligase Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm Molecular Function:acetate-CoA ligase activity; AMP binding Biological Process: ethanol oxidation; lipid biosynthetic process
NCBI Summary:	This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
UniProt Code:	Q9NR19
NCBI GenInfo Identifier:	20137525
NCBI Gene ID:	55902
NCBI Accession:	Q9NR19.1
UniProt Secondary Accession:	Q9NR19,Q5QPH2, Q5QPH3, Q8N238, Q96EL0, Q9NQP7, Q9UJ15 A6NE90,
UniProt Related Accession:	Q9NR19
Molecular Weight:	80,087 Da
NCBI Full Name:	Acetyl-coenzyme A synthetase, cytoplasmic
NCBI Synonym Full Names:	acyl-CoA synthetase short-chain family member 2
NCBI Official Symbol:	ACSS2
NCBI Official Synonym Symbols:	ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
NCBI Protein Information:	acetyl-coenzyme A synthetase, cytoplasmic
UniProt Protein Name:	Acetyl-coenzyme A synthetase, cytoplasmic
UniProt Synonym Protein Names:	Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme
Protein Family:	Acetyl-coenzyme A synthetase
UniProt Gene Name:	ACSS2
UniProt Entry Name:	ACSA_HUMAN

	Western blot analysis of extracts of various cell lines, using ACSS2 antibody (CAB13596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using ACSS2 Antibody (CAB13596) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using ACSS2 Antibody (CAB13596) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using ACSS2 antibody (CAB13596). Blue: DAPI for nuclear staining.

Antibodies

Anti-ACSS2 Antibody (CAB6472)

Anti-ACSS2 Antibody (CAB12334)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)