Anti-ACSS1 항체 (CAB15007)

Antibody Name:	Anti-ACSS1 Antibody
Antibody SKU:	CAB15007
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 340-689 of human ACSS1 (NP_115890.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-549, Mouse heart, Mouse testis, Mouse kidney, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 340-689 of human ACSS1 (NP_115890.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ITGH SYVV YGPL CNGA TSVL FEST PVYP NAGR YWET VERL KINQ FYGA PTAV RLLL KYGD AWVK KYDR SSLR TLGS VGEP INCE AWEW LHRV VGDS RCTL VDTW WQTE TGGI CIAP RPSE EGAE ILPA MAMR PFFG IVPV LMDE KGSV VEGS NVSG ALCI SQAW PGMA RTIY GDHQ RFVD AYFK AYPG YYFT GDGA YRTE GGYY QITG RMDD VINI SGHR LGTA EIED AIAD HPAV PESA VIGY PHDI KGEA AFAF IVVK DSAG DSDV VVQE LKSM VATK IAKY AVPD EILV VKRL PKTR SGKV MRRL LRKI ITSE AQEL GDTT TLED PSII AEIL SVYQ KCKD KQAA AK
Gene ID:	84532
Uniprot:	Q9NUB1
Cellular Location:	Mitochondrion matrix
Calculated MW:	62kDa/74kDa
Observed MW:	75kDa

Synonyms:	ACSS1, ACAS2L, ACECS1, AceCS2L
Background:	This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	ACSS1: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2). Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 6.2.1.1; Mitochondrial; Carbohydrate Metabolism - propanoate; Ligase Chromosomal Location of Human Ortholog: 20p11.21 Cellular Component: mitochondrial matrix Molecular Function:acetate-CoA ligase activity; AMP binding; ATP binding; protein binding Biological Process: acetate biosynthetic process; acetyl-CoA biosynthetic process; acetyl-CoA biosynthetic process from acetate; ethanol oxidation; propionate biosynthetic process; xenobiotic metabolic process
NCBI Summary:	This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:	Q9NUB1
NCBI GenInfo Identifier:	30172968
NCBI Gene ID:	84532
NCBI Accession:	Q9NUB1.2
UniProt Secondary Accession:	Q9NUB1,Q5TF42, Q8IV99, Q8N234, Q96JI1, Q96JX6, Q9NU28 B3KXL2, B4DJZ3, D3DW48, F5H6F4, F8W7Y1,
UniProt Related Accession:	Q9NUB1
Molecular Weight:	62,688 Da
NCBI Full Name:	Acetyl-coenzyme A synthetase 2-like, mitochondrial
NCBI Synonym Full Names:	acyl-CoA synthetase short-chain family member 1
NCBI Official Symbol:	ACSS1
NCBI Official Synonym Symbols:	ACAS2L; ACECS1; AceCS2L
NCBI Protein Information:	acetyl-coenzyme A synthetase 2-like, mitochondrial
UniProt Protein Name:	Acetyl-coenzyme A synthetase 2-like, mitochondrial
UniProt Synonym Protein Names:	Acetate--CoA ligase 2; Acetyl-CoA synthetase 2; AceCS2; Acyl-CoA synthetase short-chain family member 1
Protein Family:	Acetyl-coenzyme A synthetase
UniProt Gene Name:	ACSS1
UniProt Entry Name:	ACS2L_HUMAN

	Western blot analysis of extracts of various cell lines, using ACSS1 antibody (CAB15007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACSS1 Rabbit pAb (CAB15007) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using ACSS1 antibody (CAB15007) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

ACSS1 Antibody (PACO06226)

ACSS1 Antibody (PACO07280)

Acetyl-ACSS1 (K642) Antibody (PACO07427)

ACSS1 Antibody (PACO07575)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)