Anti-ACPP Antibody (CAB1937)
- SKU:
- CAB1937
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ACPP Antibody |
| Antibody SKU: | CAB1937 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human ACPP (NP_001090.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, MCF7, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human ACPP (NP_001090.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LVFR HGDR SPID TFPT DPIK ESSW PQGF GQLT QLGM EQHY ELGE YIRK RYRK FLNE SYKH EQVY IRST DVDR TLMS AMTN LAAL FPPE GVSI WNPI LLWQ PIPV HTVP LSED QLLY LPFR NCPR FQEL ESET LKSE EFQK RLHP YKDF IATL GKLS GLHG QDLF GIWS KVYD PLYC ESVH NFTL PSWA TEDT MTKL RELS ELSL LSLY GIHK QKEK SRLQ GGVL VNEI LNHM KRAT QIPS YKKL IMYS AHDT TVSG LQMA L |
| Gene ID: | 55 |
| Uniprot: | P15309 |
| Cellular Location: | Cell membrane, Lysosome membrane, Secreted, Single-pass type I membrane protein |
| Calculated MW: | 40kDa/44kDa/48kDa |
| Observed MW: | 43kDa |
| Synonyms: | ACPP, 5'-NT, ACP-3, ACP3 |
| Background: | This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. |
| UniProt Protein Function: | ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Phosphatase; EC 3.1.3.5; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q22.1 Cellular Component: multivesicular body; extracellular space; Golgi cisterna; apical part of cell; lysosomal membrane; plasma membrane; integral to membrane; vesicle membrane; intracellular; nucleus; secretory granule; filopodium Molecular Function:5'-nucleotidase activity; choline binding; identical protein binding; acid phosphatase activity; protein homodimerization activity; phosphoric monoester hydrolase activity; thiamin phosphate phosphatase activity Biological Process: thiamin metabolic process; dephosphorylation; positive regulation of adenosine receptor signaling pathway; nucleotide metabolic process; regulation of sensory perception of pain; adenosine metabolic process; purine base metabolic process |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008] |
| UniProt Code: | P15309 |
| NCBI GenInfo Identifier: | 130730 |
| NCBI Gene ID: | 55 |
| NCBI Accession: | P15309.3 |
| UniProt Related Accession: | P15309 |
| Molecular Weight: | |
| NCBI Full Name: | Prostatic acid phosphatase |
| NCBI Synonym Full Names: | acid phosphatase, prostate |
| NCBI Official Symbol: | ACPP |
| NCBI Official Synonym Symbols: | ACP3; 5'-NT; ACP-3 |
| NCBI Protein Information: | prostatic acid phosphatase |
| UniProt Protein Name: | Prostatic acid phosphatase |
| UniProt Synonym Protein Names: | 5'-nucleotidase (EC:3.1.3.5); 5'-NT; Ecto-5'-nucleotidase; Thiamine monophosphatase; TMPase |
| UniProt Gene Name: | ACPP |
| UniProt Entry Name: | PPAP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACPP antibody (CAB1937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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