Anti-ACOT9 Antibody (CAB15416)
- SKU:
- CAB15416
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ACOT9 Antibody |
| Antibody SKU: | CAB15416 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-250 of human ACOT9 (NP_001028755.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, MCF7, HT-29, Mouse brain, Mouse heart, Mouse kidney, Mouse lung, Rat pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-250 of human ACOT9 (NP_001028755.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LTQG PQNP KKQG IFHI HEVR DKLR EIVG ASTN WRDH VKAM EERK LLHS FLAK SQDG LPPR RMKD SYIE VLLP LGSE PELR EKYL TVQN TVRF GRIL EDLD SLGV LICY MHNK IHSA KMSP LSIV TALV DKID MCKK SLSP EQDI KFSG HVSW VGKT SMEV KMQM FQLH GDEF CPVL DATF VMVA RDSE NKGP AFVN PLIP ESPE EEEL FRQG ELNK GRRI AFSS TSLL K |
| Gene ID: | 23597 |
| Uniprot: | Q9Y305 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 43kDa/46kDa/49kDa/50kDa |
| Observed MW: | 50kDa |
| Synonyms: | ACOT9, ACATE2, CGI-16, MT-ACT48, MTACT48 |
| Background: | The protein encoded by this gene is a mitochondrial acyl-CoA thioesterase of unknown function. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ACOT9: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs. Belongs to the acyl coenzyme A hydrolase family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.1.2.-; Hydrolase Chromosomal Location of Human Ortholog: Xp22.11 Cellular Component: mitochondrion Molecular Function:acetyl-CoA hydrolase activity Biological Process: acyl-CoA metabolic process |
| NCBI Summary: | The protein encoded by this gene is a mitochondrial acyl-CoA thioesterase of unknown function. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| UniProt Code: | Q9Y305 |
| NCBI GenInfo Identifier: | 224471815 |
| NCBI Gene ID: | 23597 |
| NCBI Accession: | Q9Y305.2 |
| UniProt Related Accession: | Q9Y305 |
| Molecular Weight: | 50kDa |
| NCBI Full Name: | Acyl-coenzyme A thioesterase 9, mitochondrial |
| NCBI Synonym Full Names: | acyl-CoA thioesterase 9 |
| NCBI Official Symbol: | ACOT9 |
| NCBI Official Synonym Symbols: | ACATE2; CGI-16; MTACT48; MT-ACT48 |
| NCBI Protein Information: | acyl-coenzyme A thioesterase 9, mitochondrial |
| UniProt Protein Name: | Acyl-coenzyme A thioesterase 9, mitochondrial |
| UniProt Synonym Protein Names: | Acyl-CoA thioester hydrolase 9 |
| Protein Family: | Acyl-coenzyme A thioesterase |
| UniProt Gene Name: | ACOT9 |
| UniProt Entry Name: | ACOT9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACOT9 antibody (CAB15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of A431 cells using ACOT9 antibody (CAB15416) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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