Anti-ABCF2 Antibody (CAB12555)
- SKU:
- CAB12555
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ABCF2 Antibody |
| Antibody SKU: | CAB12555 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ABCF2 (NP_005683.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, 293T, HeLa, MCF7, Mouse testis, Mouse skeletal muscle, Mouse brain, Rat intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ABCF2 (NP_005683.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPSD LAKK KAAK KKEA AKAR QRPR KGHE ENGD VVTE PQVA EKNE ANGR ETTE VDLL TKEL EDFE MKKA AARA VTGV LASH PNST DVHI INLS LTFH GQEL LSDT KLEL NSGR RYGL IGLN GIGK SMLL SAIG KREV PIPE HIDI YHLT REMP PSDK TPLH CVME VDTE RAML EKEA ERLA HEDA ECEK LMEL YERL EELD ADKA EMRA SRIL HGLG FTPA MQRK KLKD FSGG WRMR VALA RALF IRPF ML |
| Gene ID: | 10061 |
| Uniprot: | Q9UG63 |
| Cellular Location: | |
| Calculated MW: | 71kDa/72kDa |
| Observed MW: | 71kDa |
| Synonyms: | ABCF2, ABC28, EST133090, HUSSY-18, HUSSY18 |
| Background: | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 7. |
| UniProt Protein Function: | ABCF2: is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This protein is a member of the GCN20 subfamily. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 7q36 Cellular Component: mitochondrial envelope; membrane; ATP-binding cassette (ABC) transporter complex Molecular Function:transporter activity; ATPase activity; ATP binding Biological Process: transport; metabolic process |
| NCBI Summary: | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding casette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 7. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9UG63 |
| NCBI GenInfo Identifier: | 12643306 |
| NCBI Gene ID: | 10061 |
| NCBI Accession: | Q9UG63.2 |
| UniProt Secondary Accession: | Q9UG63,O60864, Q75MJ0, Q75MJ1, Q96TE8, |
| UniProt Related Accession: | Q9UG63 |
| Molecular Weight: | 623 |
| NCBI Full Name: | ATP-binding cassette sub-family F member 2 |
| NCBI Synonym Full Names: | ATP-binding cassette, sub-family F (GCN20), member 2 |
| NCBI Official Symbol: | ABCF2 |
| NCBI Official Synonym Symbols: | ABC28; HUSSY18; HUSSY-18; EST133090 |
| NCBI Protein Information: | ATP-binding cassette sub-family F member 2; ABC-type transport protein; iron-inhibited ABC transporter 2 |
| UniProt Protein Name: | ATP-binding cassette sub-family F member 2 |
| UniProt Synonym Protein Names: | Iron-inhibited ABC transporter 2 |
| Protein Family: | ABC transporter F family |
| UniProt Gene Name: | ABCF2 |
| UniProt Entry Name: | ABCF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ABCF2 antibody (CAB12555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using ABCF2 antibody (CAB12555) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using ABCF2 antibody (CAB12555) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using ABCF2 antibody (CAB12555) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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