Anti-ABCC2 Antibody (CAB8405)
- SKU:
- CAB8405
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-ABCC2 Antibody |
| Antibody SKU: | CAB8405 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-315 of human ABCC2 (NP_000383.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HeLa+PNGaseF |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-315 of human ABCC2 (NP_000383.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IFSA FSEN NESS NNPS SIAS FLSS ITYS WYDS IILK GYKR PLTL EDVW EVDE EMKT KTLV SKFE THMK RELQ KARR ALQR RQEK SSQQ NSGA RLPG LNKN QSQS QDAL VLED VEKK KKKS GTKK DVPK SWLM KALF |
| Gene ID: | 1244 |
| Uniprot: | Q92887 |
| Cellular Location: | Apical cell membrane, Multi-pass membrane protein |
| Calculated MW: | 174kDa |
| Observed MW: | 300kDa |
| Synonyms: | ABCC2, ABC30, CMOAT, DJS, MRP2, cMRP |
| Background: | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. |
| UniProt Protein Function: | ABCC2: Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Transporter, ABC family; Membrane protein, integral; Transporter Chromosomal Location of Human Ortholog: 10q24 Cellular Component: intercellular canaliculus; cell surface; integral to plasma membrane; apical plasma membrane; plasma membrane Molecular Function:protein domain specific binding; protein binding; ATPase activity, coupled to transmembrane movement of substances; organic anion transmembrane transporter activity; ATP binding Biological Process: prostaglandin transport; metabolic process; response to heat; response to estrogen stimulus; transport; response to arsenic; multidrug transport; response to methotrexate; response to oxidative stress; cellular chloride ion homeostasis; transmembrane transport Disease: Dubin-johnson Syndrome |
| NCBI Summary: | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92887 |
| NCBI GenInfo Identifier: | 4557481 |
| NCBI Gene ID: | 1244 |
| NCBI Accession: | NP_000383.1 |
| UniProt Related Accession: | Q92887 |
| Molecular Weight: | |
| NCBI Full Name: | canalicular multispecific organic anion transporter 1 |
| NCBI Synonym Full Names: | ATP binding cassette subfamily C member 2 |
| NCBI Official Symbol: | ABCC2 |
| NCBI Official Synonym Symbols: | DJS; MRP2; cMRP; ABC30; CMOAT |
| NCBI Protein Information: | canalicular multispecific organic anion transporter 1 |
| UniProt Protein Name: | Canalicular multispecific organic anion transporter 1 |
| UniProt Synonym Protein Names: | ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Multidrug resistance-associated protein 2 |
| Protein Family: | 37S ribosomal protein |
| UniProt Gene Name: | ABCC2 |
| UniProt Entry Name: | MRP2_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using ABCC2 antibody (CAB8405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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