Anti-AATF Antibody (CAB5896)
- SKU:
- CAB5896
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-AATF Antibody |
| Antibody SKU: | CAB5896 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 7-100 of human AATF (NP_036270.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 7-100 of human AATF (NP_036270.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LALQ LEQL LNPR PSEA DPEA DPEE ATAA RVID RFDE GEDG EGDF LVVG SIRK LASA SLLD TDKR YCGK TTSR KAWN EDHW EQTL PGSS DEEI SD |
| Gene ID: | 26574 |
| Uniprot: | Q9NY61 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 63kDa |
| Observed MW: | 63kDa |
| Synonyms: | AATF, BFR2, CHE-1, CHE1, DED |
| Background: | The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. |
| UniProt Protein Function: | AATF: May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. Belongs to the AATF family. |
| UniProt Protein Details: | Protein type:Nucleolus; RNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 17q12 Cellular Component: centrosome; cytoplasm; focal adhesion; Golgi apparatus; nucleolus; nucleus Molecular Function:leucine zipper domain binding; protein binding; tau protein binding; transcription factor activity Biological Process: cell adhesion; embryonic cleavage; negative regulation of amyloid precursor protein biosynthetic process; negative regulation of apoptosis; negative regulation of superoxide release; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of transcription from RNA polymerase II promoter; regulation of mitotic cell cycle; response to DNA damage stimulus; ribosome biogenesis and assembly |
| NCBI Summary: | The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NY61 |
| NCBI GenInfo Identifier: | 7657013 |
| NCBI Gene ID: | 26574 |
| NCBI Accession: | NP_036270.1 |
| UniProt Secondary Accession: | Q9NY61,Q9P0A4, Q9UNX5, A6NCJ6, B3KQ26, |
| UniProt Related Accession: | Q9NY61 |
| Molecular Weight: | 58.8 kDa |
| NCBI Full Name: | protein AATF |
| NCBI Synonym Full Names: | apoptosis antagonizing transcription factor |
| NCBI Official Symbol: | AATF |
| NCBI Official Synonym Symbols: | DED; BFR2; CHE1; CHE-1 |
| NCBI Protein Information: | protein AATF |
| UniProt Protein Name: | Protein AATF |
| UniProt Synonym Protein Names: | Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1 |
| Protein Family: | Protein |
| UniProt Gene Name: | AATF |
| UniProt Entry Name: | AATF_HUMAN |
 | Western blot analysis of extracts of mouse brain, using AATF antibody (CAB5896). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
Related Products
