Anti-AADAC Antibody (CAB13936)
- SKU:
- CAB13936
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-AADAC Antibody |
| Antibody SKU: | CAB13936 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-399 of human AADAC (NP_001077.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Rat liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-399 of human AADAC (NP_001077.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KKVL AKYG VNPE RIGI SGDS AGGN LAAA VTQQ LLDD PDVK IKLK IQSL IYPA LQPL DVDL PSYQ ENSN FLFL SKSL MVRF WSEY FTTD RSLE KAML SRQH VPVE SSHL FKFV NWSS LLPE RFIK GHVY NNPN YGSS ELAK KYPG FLDV RAAP LLAD DNKL RGLP LTYV ITCQ YDLL RDDG LMYV TRLR NTGV QVTH NHVE DGFH GAFS FLGL KISH RLIN QYIE WLKE NL |
| Gene ID: | 13 |
| Uniprot: | P22760 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane, Single-pass type II membrane protein |
| Calculated MW: | 45kDa |
| Observed MW: | 46kDa |
| Synonyms: | AADAC, CES5A1, DAC |
| Background: | Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens |
| UniProt Protein Function: | AADAC: Arylacetamide deacetylation is an important enzyme activity in the metabolic activation of arylamine substrates to ultimate carcinogens. Displays major serine hydrolase activity in liver microsomes. Hydrolyzes also flutamide, which is an antiandrogen drug used for the treatment of prostate cancer that occasionaly causes severe hepatotoxicity. Displays cellular triglyceride lipase activity in liver. Increases intracellular fatty acids derived from hydrolysis of newly formed triglyceride stores. Belongs to the 'GDXG' lipolytic enzyme family. |
| UniProt Protein Details: | Protein type:Deacetylase; Endoplasmic reticulum; Motility/polarity/chemotaxis; Lipase; Membrane protein, integral; EC 3.1.1.3 Chromosomal Location of Human Ortholog: 3q25.1 Cellular Component: endoplasmic reticulum membrane Molecular Function:catalytic activity; deacetylase activity; lipase activity; serine hydrolase activity; triacylglycerol lipase activity Biological Process: xenobiotic metabolic process |
| NCBI Summary: | Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22760 |
| NCBI GenInfo Identifier: | 317373587 |
| NCBI Gene ID: | 13 |
| NCBI Accession: | P22760.5 |
| UniProt Secondary Accession: | P22760,Q8N1A9, A8K3L3, D3DNJ6, |
| UniProt Related Accession: | P22760 |
| Molecular Weight: | 45,734 Da |
| NCBI Full Name: | Arylacetamide deacetylase |
| NCBI Synonym Full Names: | arylacetamide deacetylase |
| NCBI Official Symbol: | AADAC |
| NCBI Official Synonym Symbols: | DAC; CES5A1 |
| NCBI Protein Information: | arylacetamide deacetylase |
| UniProt Protein Name: | Arylacetamide deacetylase |
| Protein Family: | Arylacetamide deacetylase |
| UniProt Gene Name: | AADAC |
| UniProt Entry Name: | AAAD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AADAC antibody (CAB13936) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunofluorescence analysis of 293T cells using AADAC antibody (CAB13936) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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