Anti-AADAC Antibody (CAB10365)

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SKU:
CAB10365
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-AADAC Antibody
Antibody SKU:CAB10365
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 170-399 of human AADAC (NP_001077.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:A-549, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 170-399 of human AADAC (NP_001077.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KKVL AKYG VNPE RIGI SGDS AGGN LAAA VTQQ LLDD PDVK IKLK IQSL IYPA LQPL DVDL PSYQ ENSN FLFL SKSL MVRF WSEY FTTD RSLE KAML SRQH VPVE SSHL FKFV NWSS LLPE RFIK GHVY NNPN YGSS ELAK KYPG FLDV RAAP LLAD DNKL RGLP LTYV ITCQ YDLL RDDG LMYV TRLR NTGV QVTH NHVE DGFH GAFS FLGL KISH RLIN QYIE WLKE NL
Gene ID:13
Uniprot:P22760
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Single-pass type II membrane protein
Calculated MW:45kDa
Observed MW:46kDa
Synonyms:AADAC, CES5A1, DAC
Background:Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens
UniProt Protein Function:AADAC: Arylacetamide deacetylation is an important enzyme activity in the metabolic activation of arylamine substrates to ultimate carcinogens. Displays major serine hydrolase activity in liver microsomes. Hydrolyzes also flutamide, which is an antiandrogen drug used for the treatment of prostate cancer that occasionaly causes severe hepatotoxicity. Displays cellular triglyceride lipase activity in liver. Increases intracellular fatty acids derived from hydrolysis of newly formed triglyceride stores. Belongs to the 'GDXG' lipolytic enzyme family.
UniProt Protein Details:

Protein type:Deacetylase; Endoplasmic reticulum; Motility/polarity/chemotaxis; Lipase; Membrane protein, integral; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: endoplasmic reticulum membrane

Molecular Function:catalytic activity; deacetylase activity; lipase activity; serine hydrolase activity; triacylglycerol lipase activity

Biological Process: xenobiotic metabolic process

NCBI Summary:Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens [provided by RefSeq, Jul 2008]
UniProt Code:P22760
NCBI GenInfo Identifier:317373587
NCBI Gene ID:13
NCBI Accession:P22760.5
UniProt Secondary Accession:P22760,Q8N1A9, A8K3L3, D3DNJ6,
UniProt Related Accession:P22760
Molecular Weight:45,734 Da
NCBI Full Name:Arylacetamide deacetylase
NCBI Synonym Full Names:arylacetamide deacetylase
NCBI Official Symbol:AADAC  
NCBI Official Synonym Symbols:DAC; CES5A1  
NCBI Protein Information:arylacetamide deacetylase
UniProt Protein Name:Arylacetamide deacetylase
Protein Family:Arylacetamide deacetylase
UniProt Gene Name:AADAC  
UniProt Entry Name:AAAD_HUMAN
Anti-AADAC Antibody (CAB10365)Western blot analysis of extracts of various cell lines, using AADAC antibody (CAB10365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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