Alpha-Mannosidase Activity Assay Kit (BA0014)

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SKU:
BA0014
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Biological Samples Plasma, Serum, Tissue, Culture Media
Research Area:
Plant & Environmental Stress
Enzyme Activity
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

alpha-Mannosidase Activity Assay Kit - Information

Assay Genie's non-radioactive, colorimetric alpha-Mannosidase activity assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Applications

For quantitative determination of alpha-mannosidase enzyme activity using a p-nitrophenol based substrate.

alpha-Mannosidase Activity Assay Kit - Key Features

  • Fast and sensitive. Linear detection range (10 sample): 1 to 250 U/L for a 10 minute reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day.

alpha-Mannosidase Activity Assay Kit - Data Sheet

Kit IncludesSubstrate Buffer: 10 mL Stop Reagent: 12 mL Standard: 1 mL 12.5 mM Nitrophenol
Kit RequiresPipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, centrifuge tubes and plate reader.
Method of DetectionOD405nm
Detection Limit1 U/L
SamplesBiological samples e.g. plasma, serum, tissue and culture media.
SpeciesAll
Protocol Length10 min
Size100 tests
StorageStore all components at 4°C upon receiving.
Shelf Life1 U/L

More Details

alpha-MANNOSIDASE (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. alpha-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes alpha-mannosidosis and leads to deterioration of the central nervous system in children.

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