ALOX15B Antibody (PACO24880)
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주문- SKU:
- PACO24880
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | ALOX15B Antibody (PACO24880) |
Antibody SKU: | PACO24880 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Arachidonate 15-lipoxygenase B protein (1-676AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot All lanes: ALOX15B antibody at 16µg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 68, 70, 73 kDa Observed band size: 76 kDa . |
![]() | Immunofluorescence staining of A549 cells with PACO24880 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acid, generating a spectrum of bioactive lipid mediators. Converts arachidonic acid, to 15S-hydroperoxyeicosatetraenoic acid, (15S)-HPETE. Also acts on linoleic acid, to produce 13-hydroxyoctadecadienoic acid, 13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells. |
Synonyms: | Arachidonate 15-lipoxygenase B (15-LOX-B) (EC 1.13.11.33) (15-lipoxygenase 2) (15-LOX-2) (Arachidonate 15-lipoxygenase type II) (Linoleate 13-lipoxygenase 15-LOb) (EC 1.13.11.-), ALOX15B |
UniProt Protein Function: | ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; EC 1.13.11.33; Apoptosis; Lipid Metabolism - arachidonic acid; Cell cycle regulation; Oxidoreductase Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: cytoplasm; cytoskeleton; cytosol; extrinsic to membrane; intracellular; membrane; plasma membrane Molecular Function:arachidonate 15-lipoxygenase activity; calcium ion binding; iron ion binding; lipoxygenase activity Biological Process: apoptosis; arachidonic acid metabolic process; hepoxilin biosynthetic process; lipid metabolic process; lipoxygenase pathway; negative regulation of cell cycle; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of growth |
NCBI Summary: | This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
UniProt Code: | O15296 |
NCBI GenInfo Identifier: | 317373425 |
NCBI Gene ID: | 247 |
NCBI Accession: | O15296.3 |
UniProt Secondary Accession: | O15296,Q8IYQ2, Q8TEV3, Q8TEV4, Q8TEV5, Q8TEV6, Q9UKM4 D3DTR2, |
UniProt Related Accession: | O15296 |
Molecular Weight: | 72,523 Da |
NCBI Full Name: | Arachidonate 15-lipoxygenase B |
NCBI Synonym Full Names: | arachidonate 15-lipoxygenase, type B |
NCBI Official Symbol: | ALOX15B |
NCBI Official Synonym Symbols: | 15-LOX-2 |
NCBI Protein Information: | arachidonate 15-lipoxygenase B |
UniProt Protein Name: | Arachidonate 15-lipoxygenase B |
UniProt Synonym Protein Names: | 15-lipoxygenase 2; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb (EC:1.13.11.-) |
Protein Family: | Arachidonate 15-lipoxygenase |
UniProt Gene Name: | ALOX15B |
UniProt Entry Name: | LX15B_HUMAN |
Antibodies | ELISA Kits |
Anti-ALOX15B Antibody (CAB6865) | Human Arachidonate 15-lipoxygenase B / ALOX15B ELISA Kit |
ALOX15B Antibody (PACO07704) | Human Arachidonate 15-lipoxygenase B (ALOX15B) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |