Alkaline Phosphatase Assay Kit (Colorimetric) (BA0013)

Alkaline Phosphatase Assay - Information

Alkaline Phosphatase Assay Kit (Colorimetric) is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

Applications

For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate.

Alkaline Phosphatase Assay - Key Features

• High sensitivity and wide linear range. Use 5 serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L.
• Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes.
• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Alkaline Phosphatase Assay - Data Sheet
Kit Includes	Assay Buffer: 50 mL, pH 10.5 Mg Acetate: 1.5 mL 0.2 M pNPP Liquid: 600 mL 1 M Calibrator: 10 mL Tartrazine
Kit Requires	Pipeting devices and accessories (e.g. multi-channel pipettor)
Method of Detection	OD405nm
Detection Limit	2 U/L
Samples	Serum, plasma etc
Species	All
Protocol Length	4 min
Size	250 tests
Storage	Store pNPP Liquid and Calibrator at -20°C and other components at 4°C.
Shelf Life	12 months

More Details

Alkaline phosphatase(ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery.

Aliases for ALPL Gene

• AlkalinePhosphatase, Liver/Bone/Kidney
• AlkalinePhosphataseLiver/Bone/Kidney Isozyme
• EC 3.1.3.1
• AP-TNAP
• TNSALP
• AlkalinePhosphatase, Tissue-Nonspecific Isozyme

Entrez Gene Summary for ALPL Gene

This gene encodes a member of thealkalinephosphatasefamily of proteins. There are at least four distinct but relatedalkalinephosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]

UniProtKB/Swiss-Prot for ALPL Gene

PPBT_HUMAN,P05186

• This isozyme may play a role in skeletal mineralization

Protein details for ALPL Gene (UniProtKB/Swiss-Prot)

Protein Symbol:P05186-PPBT_HUMANRecommended name:Alkalinephosphatase, tissue-nonspecific isozymeProtein Accession:P05186Secondary Accessions:A1A4E7, B2RMP8, B7Z387, B7Z4Y6, O75090, Q2TAI7, Q59EJ7, Q5BKZ5, Q5VTG5, Q6NZI8, Q8WU32, Q9UBK0

Protein attributes for ALPL Gene

Size:524 amino acidsMolecular mass:57305 DaCofactor:Name=Mg(2+); Xref=ChEBI:CHEBI:18420;Cofactor:Name=Zn(2+); Xref=ChEBI:CHEBI:29105;Quaternary structure:Homodimer.SequenceCaution:Sequence=BAD93051.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

Post-translational modifications for ALPL Gene

• N-glycosylated.P05186-PPBT_HUMAN
• Glycosylation at Asn 140, Asn 230, Asn 271, Asn 303, and Asn 430NX_P05186
• Modification sites at PhosphoSitePlusP05186