Alanine Transaminase Assay Kit (BA0074)

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SKU:
BA0074
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma
Research Area:
Diabetes & Obesity
Amino Acid and Protein
Clinical Chemistry
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Alanine Transaminase Assay Kit - Information

Assay Genie's alanine transaminase activity assay (ALT) is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity.

Applications

For quantitative determination of alanine transaminase activity.

Alanine Transaminase Assay Kit - Key Features

  • Sensitive. Linear detection range: 2-100 U/L.
  • Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.

Alanine Transaminase Assay Kit - Data Sheet

Kit IncludesAssay Buffer: 24 mL LDH: 120 uL Cosubstrate: 600 uL NADH Reagent: Dried
Kit RequiresPipeting devices and accessories. Clear bottom 96-well plates and plate reader or spectrophotometer and cuvettes for measuring OD340nm.
Method of DetectionOD340nm
Detection Limit2 U/L
SamplesSerum, plasma etc
SpeciesAll
Protocol Length10 min
Size100 tests
StorageStore all components at -20°C
Shelf Life6 months

More Details

Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery.

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