The AF/L.E. Purified Mouse H-2 Monoclonal Antibody (M1/42.3.9.8) (AGEL2108) is a specialized tool for researchers studying immune responses and histocompatibility. This monoclonal antibody, derived from mouse cells, offers high specificity and sensitivity for detecting and analyzing H-2 antigens in experimental settings. H-2 antigens are essential for understanding the immune system's recognition of self and non-self cells, playing a crucial role in immune response regulation and transplantation compatibility. The AF/L.E. purified antibody specifically targets the H-2 antigens, facilitating their identification in various biological samples with precision.
By utilizing the AF/L.E. Purified Mouse H-2 Monoclonal Antibody (M1/42.3.9.8) (AGEL2108), researchers can delve deeper into the mechanisms of immune recognition, histocompatibility, and immune cell interactions. This antibody serves as a valuable asset in immunology research, transplantation studies, and investigations related to immune-mediated diseases. Its reliability and reproducibility make it an indispensable tool for unraveling the complexities of the immune system and its implications in health and disease.
Mouse major histocompatibility complex (MHC) H-2; MHC I;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM;Block
Isotype:
Rat IgG2a, κ
Isotype Control:
AF/LE Purified Rat IgG2a, κ Isotype Control[2A3]
Form:
Liquid
Conjugation:
None (AF/LE)
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Gene ID:
111364
Storage Buffer:
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.