The Mouse CD25 Monoclonal Antibody (PC61.5.3) offered by Assay Genie (AGEL1296) is a highly purified antibody designed for research applications involving CD25, a critical cell surface marker associated with regulatory T cells. This monoclonal antibody, developed specifically for mouse samples, is ideal for use in various immunoassays, flow cytometry, and immunohistochemistry studies.CD25, also known as interleukin-2 receptor alpha chain, plays a crucial role in immune regulation by facilitating the activation and proliferation of regulatory T cells. The PC61.5.3 antibody specifically targets CD25, enabling precise detection and analysis of its expression in different cell types and tissues.
This antibody is essential for investigating the function of regulatory T cells in immune tolerance, autoimmunity, and anti-tumor immunity.Researchers and scientists studying immunology, cancer biology, and autoimmune diseases can benefit from the high specificity and purity of the Mouse CD25 Monoclonal Antibody (PC61.5.3). By using this antibody, they can gain valuable insights into the mechanisms underlying immune tolerance and immune-mediated diseases, paving the way for the development of novel therapeutic strategies targeting CD25 and regulatory T cells.
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.