The AF/b – LE Purified Mouse CD16/32 Monoclonal Antibody (2.4G2) - AGEL0576 is an essential tool for researchers studying immune responses and cell signaling pathways. This monoclonal antibody, derived from mouse cells, specifically targets the CD16/32 protein, which is known for its role in mediating cellular functions in the immune system. With high specificity and sensitivity, this antibody is ideal for various applications such as flow cytometry, immunoprecipitation, and immunofluorescence.CD16/32, also referred to as Fc gamma receptor III and II, is a key player in immune system regulation, particularly in antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis.
By targeting this protein, researchers can gain valuable insights into the mechanisms underlying immune responses, autoimmune disorders, and infectious diseases. This antibody enables precise detection and analysis of CD16/32 expression in different cell types, paving the way for advancements in immunology research and drug development.
CD16a/b;CD32;CD32A/B;Fc fragment of IgG low affinity IIa/b receptor;Fc fragment of IgG low affinity IIIa/b receptor;Fc fragment of IgG low affinity IIIb receptor;Fc gamma receptor III A/B;FCG2A;FcGR;FCGR2A/BFCGR3;FCGR3A/B;Fc gamma RIIa/b ;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM;Block
Isotype:
Rat IgG2b, κ
Isotype Control:
AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
None (AF/LE)
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Swissprot:
P08508 P08101
Gene ID:
14130,14131
Storage Buffer:
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.