The IFN Gamma Monoclonal Antibody (B27) is a purified human antibody designed for research applications involving interferon-gamma, a crucial cytokine involved in immune responses and inflammation regulation. This monoclonal antibody, known as B27, is highly specific and reactive with human samples, making it a reliable tool for various immunoassays, including immunofluorescence, flow cytometry, and ELISA.Interferon-gamma is a key player in host defense mechanisms, exerting both pro-inflammatory and immunoregulatory effects. By targeting and detecting interferon-gamma, researchers can gain insights into immune system function, pathogen defense, autoimmune diseases, and cancer immunity.
This monoclonal antibody binds specifically to interferon-gamma, enabling precise detection and analysis in diverse experimental settings.Utilizing the IFN Gamma Monoclonal Antibody (B27) in your research allows for the elucidation of intricate immune responses and the evaluation of interferon-gamma expression levels in various biological samples. Whether investigating immune-related disorders, studying immune cell interactions, or exploring therapeutic interventions, this antibody serves as a valuable tool for advancing immunological research and drug development efforts.
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is between 0.25-2 µg/mL per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Swissprot:
P01579
Gene ID:
3458
Storage Buffer:
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.