The AF-LE Purified Human CD28 Monoclonal Antibody (CD28-2) is a vital tool for researchers studying the CD28 protein, a key regulator of T cell activation and function. This monoclonal antibody, produced with high purity and specificity, is ideal for use in various immunology studies and cancer research projects.CD28 is a co-stimulatory molecule found on the surface of T cells that plays a crucial role in enhancing immune responses. By binding specifically to CD28, this antibody allows for the detection and analysis of this important protein in different cell types, providing valuable insights into T cell activation and signaling pathways.The AF-LE Purified Human CD28 Monoclonal Antibody is validated for use in applications such as flow cytometry, immunofluorescence, and ELISA, enabling researchers to investigate the role of CD28 in immune regulation and response modulation.
Its high reactivity with human samples makes it a reliable tool for exploring potential therapeutic targets in diseases like cancer, autoimmune disorders, and inflammatory conditions.By utilizing this antibody in their research, scientists can gain a deeper understanding of the mechanisms underlying T cell activation and immune regulation, paving the way for the development of innovative therapies and treatment strategies in various disease settings.
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Swissprot:
P10747
Gene ID:
940
Storage Buffer:
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
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