The 8-OHdG (8-hydroxydeoxyguanosine) ELISA Kit is specifically designed for the accurate quantification of 8-OHdG levels in biological samples such as urine, plasma, serum, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.8-OHdG is a well-known marker of oxidative DNA damage and is commonly used in studying oxidative stress-related diseases such as cancer, cardiovascular diseases, and aging.
By accurately measuring 8-OHdG levels, researchers can gain valuable insights into the pathogenesis of these diseases and potentially develop novel therapeutic strategies. Overall, the 8-OHdG ELISA Kit provides researchers with a powerful tool for investigating oxidative stress, biomarker discovery, and drug development in a reliable and efficient manner.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-OHdG. During the reaction, 8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-OHdG in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
87-101
90-103
Average (%)
98
92
95
1:4
Range (%)
89-106
82-94
83-95
Average (%)
97
88
90
1:8
Range (%)
93-106
85-100
88-99
Average (%)
100
92
94
1:16
Range (%)
87-102
86-99
88-101
Average (%)
94
92
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
91-104
nan
98.0
nan
nan
nan
Mean (ng/mL)
93-106
nan
98.0
nan
nan
nan
Standard deviation
20
20.0
20.0
20.0
20.0
20.0
C V (%)
4.79
10.11
46.98
4.48
9.61
48.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of 8-OHdG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes 8-OHdG in samples. No significant cross-reactivity or interference between 8-OHdG and analogues was observed.
